Page 7 - Shimadzu LCMS-8050

UFscanning

UFsensitivity

UFswitching
UF-MRM

Heated ESI Probe
Heating gas flow Nebulizer gas flow
In order to improve desolvation efficiency, the newly developed heated ESI probe
combines a high-temperature gas with the nebulizer spray, assisting in the
desolvation of large droplets and facilitating ionization. This development allows * *
for high-sensitivity analysis of a wide range of target compounds.

100
289.05 > 97.10
*The transmission of
Heating gas ON heat to the LC line
has been minimized
Signal intensity: by mounting the
%
30 X improvement heaters in the
bottom-most
position.
Heating gas OFF
0
0.50 1.00 1.50 min
MRM Chromatogram of Testosterone

Excellent Reproducibility Even at Attogram (ag) Levels

Both sensitivity and reproducibility are essential when establishing low limits of
quantitation. High-precision quantitative results obtained with the LCMS-8050 in 100 455.4> 165.0
500 ag in plasma
the analysis of Verapamil in blood plasma at levels between 500 ag and 50 pg are
shown below. Excellent reproducibility with a % RSD of 2.77 % was obtained when
analyzing just 500 ag of Verapamil. The LCMS-8050 demonstrates optimal
performance for quantitative analysis of even trace components of a complex matrix.

%
Concentration Calculated % RSD Accuracy
actual concentration (%)
ng/mL ng/mL (n = 6) (n = 6)
0.000500 0.000501 2.77 100.2
0.00500 0.00496 3.98 99.2
0.0500 0.0506 1.21 101.2
0.500 0.493 1.31 98.6 0
5.00 4.89 1.81 97.8 0.8 0.9 1.0 1.1 1.2 min
50.0 51.6 0.65 103.2 MRM Chromatogram of Verapamil

Liquid Chromatograph Mass Spectrometer /&06 7