Page 4 - Shimadzu LCMS-8050

Experience a New Realm of

High-Sensitivity & High-Speed Performance

UFsensitivity ™

Combining Speed and High Sensitivity

UFswitching ™ High-Sensitivity & High-Speed Positive/Negative Ionization Switching in 5 msec

A Case Study Using High-Speed Positive/Negative Ionization Switching

High-speed polarity switching has a high impact on LC-MS/MS method design and capability by optimizing the signal response for each target
compound resulting in a single injection analysis cycle, faster rates of sample throughput and a greater number of target compounds in a single
method.

Existing CYP Inhibition Method: 7 Minutes High-speed polarity switching pushes the boundaries further;
100
Supports high data sampling rates delivering over 20 points across a UHPLC peak
% Reduce to 1 minute Improves reproducibility even at the lowest calibration level
Enhanced dynamic range compared to other triple quadrupoles
0
0.0 1 min 2.0 3.0 4.0 5.0 6.0 7 min
Legacy HPLC Method Nexera with LCMS-8050
7 min 1 min
Nexera™ with LCMS-8050 Compound Polarity
%RSD
%RSD
100 Dynamic Points/ 0.6 nmol/L Dynamic Points/ 0.6 nmol/L
range
range
peak
peak
(nmol/L) (n=4) (nmol/L) (n=4)
Resorufin 0.6-300 19 4.66 0.6-1000 21 4.30
%
1’-Hydroxy Bufuralol 0.6-300 21 2.39 0.6-1000 24 1.82
(+/-)-4’-Hydroxy Mephenytoin 0.6-300 20 2.75 0.6-1000 23 2.18
Oxidized Nifedipine 0.6-300 19 5.58 0.6-1000 23 5.07
0
0.00 0.25 0.50 0.75 1 min Hydroxy Tolbutamide 0.6-300 20 5.68 0.6-1000 23 2.96
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