Page 2 - Shimadzu Tm Analysis System
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Tm Analysis System









                     Tm analysis systems can accelerate the development process and improve the

                     quality of oligonucleotide therapeutics. Control by LabSolutions  software enables
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                     compliance with ER/ES-related regulatory requirements and improves the
                     efficiency of analyzing the thermal stability (Tm analysis) of nucleic acids.













                     Reliable Data Integrity

                     The Tm analysis system in conjunction with LabSolutions DB/CS can achieve the highest data integrity
                     levels in the industry. Measurement parameters, audit trails, and results of data acquisition and analysis
                     are managed in a database protected with user privilege settings and security policies to prevent
                     unintended operations or data tampering by operators.








                     Functionality for Satisfying Various Needs
                                                                                                                                        Tm Analysis
                     In addition to trace measurement and high-sensitivity measurement capabilities required for Tm analysis,
                     the system also satisfies a variety of other needs, including thermodynamic parameter analysis using                                                                                               Melting Curve
                                                                                                                                        Tm analysis serves an important role in checking the
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                     functionality for automatically transferring data to an Excel  file.
                                                                                                                                        thermal stability and sequence of nucleic acids. In Tm                        Single-Strand
                                                                                                                                        analysis, heat is applied to the nucleic acids in
                                                                                                                                        double-strands. Then, the change in absorbance      Double-Strand
                                                                                                                                        (melting curve) that occurs as the temperature increases   Absorbance at 260 nm (Abs)  Tm Value
                                                                                                                                        and the strands dissociate into single strands is
                     Efficient Automated Workflow                                                                                         measured. The melting temperature (Tm value) is

                     Tm analysis system dramatically decreases the time and trouble required for Tm analysis by automating all          determined as the temperature where the mole
                     steps (particularly the time-consuming annealing and analysis steps).                                              fractions of single and double strands are equal.                 Temperature



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                     Note: The photo on the right shows the UV-2600i ultraviolet-visible spectrophotometer with a TMSPC -8i 8-cell thermoelectrically temperature-controlled cell
                     holder connected. However, the TMSPC-8i can be also connected to either UV-1900i or UV-2700i models.
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