Page 9 - Shimadzu Prominence

LC-20AP
Supporting Analytical and Preparative Scales
The high solvent delivery performance of the LC-20AP permits an accurate and efficient environment for scaling up,
in both the preparative and analytical flow rate ranges. The Prominence preparative system supports both analysis
and fractionation (including gradient analysis and fractionation), which permits efficient scaling up with a single
instrument and single operating environment. Combining the LC-20AP with a dedicated FCV-200AL low-pressure
gradient unit allows gradient fractionation to be performed in a single unit, thereby saving equipment costs.

Single System Supports Parameter Investigation and Scaling Up

mV
6
Analytical injector 800 A) Analytical 1 4 7 8
700 2
Solvent delivery units Analytical mixer Analytical column 3 5
600
500
Column-switching valve 400
300 B) Preparative
UV-VIS detector
200
100
0
0 2 4 6 8 10 12 14 min
1: Benzoic acid 5: Biphenyl Column : A) 250 mmL. × 4.6 mmI.D.
Preparative mixer Preparative column Data Fraction 2: 2-naphthol 6: Phenanthrene B) 250 mmL. × 20 mmI.D.
(5 μm, same packing material)
3: Benzene
7: Anthracene
Preparative injector processing collector 4: Naphthalene 8: Fluoranthene
unit
Example of Scale-up with the Column of the Same Packing Material
High-Precision Delivery Performance
mV
A redesigned plunger actuation mechanism and Samples
1 2 3 4 (retention time at %RSD n = 6)
improved pulse correction and check valves offers 1200 1: Methylparaben
1000
significantly improved flow rate accuracy and flow rate (0.015%RSD)
800
precision. High retention time reproducibility for 2: Ethylparaben
600
(0.011%RSD)
analytical flow rates improves reliability when scaling up 400 3: Propylparaben
and verifying purity. 200 (0.008%RSD)
0 4: Butylparaben
(0.010%RSD)
1 2 3 4 5 6 7 8 9 10 min
Flow rate: 1.0 mL/min
Retention Time Reproducibility for Analytical Flow Rates
LC-20AP Quaternary Enables Fractionation as a Low-Pressure Gradient System
The LC-20AP Quaternary flow rate range is 1 to 50 mL/min and the instrument can handle up to four mobile phases simultaneously.
It considerably reduces the effort required to investigate mobile phases during method development.nt.
me
p
mAU
, 15 μ
m
.
Column Shim-pack PREP-ODS 250 mmL. × 20 mmI.D., 15 μm
Rosmarinic acid Carnosol Carnosic acid Gradient program B. Conc. 30 % (0 min) to 95 % (15 to 30 min) C. Conc. 5 %%
acid solution
1500 Mobile phases A: Water B: Methanol C: 2 % aqueous formic acid solution
C
. Conc. 5
1000 Column temperature Room temperature
Injection volume 200 μL
500
Flow rate 20.0 mL/min
0 Detection UV 230 nm
Sample Rosemary extract
0.0 5.0 10.0 15.0 20.0 25.0 min
Degassing Units DGU-20A3R / 20A5R
The DGU-20A3R/20A5R is an on-line degassing unit that uses fluoroethylene membrane. The internal
nternal
capacity is small at 0.4 mL, only 1/25th of that for existing Shimadzu models, and the waiting time at
mobile-phase replacement or stabilization can be significantly reduced. The degassing efficiency has
also been improved, ensuring thorough degassing even at high flow rates.
Number of degassed solvents for DGU-20A3R : 3
Number of degassed solvents for DGU-20A5R : 5
9
High Performance Liquid Chromatograph