Page 11 - Shimadzu Prominence

Rack Changer : Increasing the Number of Processed Samples

A rack changer is an optional product that can be used to change the microplates in the
autosampler's racks and thereby facilitate serial analysis. Up to 12 plates can be mounted
in the rack changer.
This model incorporates a cooling function. When the samples have been prepared, simply
set them in the rack changer to perform continuous sample processing.

Rack changer II (228-45164-XX) Compatible plates: 96-well MTPs, 96-well DWPs
384-well MTPs, 384-well DWPs
1.5 mL vial plate (54 vials)
Number of processed plates: 12
Sample cooler: Block cooling/heating,
used together with dehumidifying function, 4 to 40°C

Prominence UFLC Offers Ultra Fast HPLC Analysis

The SIL-20AHT / 20ACHT autosamplers used 240 mAU
Retention Time Area
with the Prominence UFLC system permit Components Average %RSD Average %RSD
ultra-fast analysis while maintaining the 200
Acetophenone 0.916 0.089 312,670 0.059
injection accuracy and durability requirements Propiophenone 1.398 0.058 315,739 0.045
150
demanded of an HPLC system.
Butyrophenone 2.111 0.030 336,428 0.063
As shown in the right, the retention time 3.341 0.031 296,609 0.040
100 Valerophenone
reproducibility and injection volume (5 μL injection, n=6)
reproducibility are within 0.3 % after 50
endurance testing over 100,000 cycles, results
similar to those achieved with standard HPLC. 0
−10
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 min
Repeatability for 4 Alkyl Phenones After
Finishing 100,000 Cycle Endurance Test

Combination with LCMS for Ultra Fast Analysis

Combining Prominence UFLC with a Shimadzu Detection : ESI-Positive mode; LCMS-2020
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mass spectrometer, which features ultra-fast Column : Shim-pack XR-ODS (30 mmL. × 3.0 mmI.D.)
2 Mobile phase : A) 0.1% aqueous solution of formic acid
polarity switching between positive/negative B) Acetonitrile
1
ion modes (UFswitching), superb sensitivity due 3 B.Conc.: 20% (0 min.) 35% (2.0 min.)
Flow rate : 0.5 mL/min.
to the newly-developed Qarray ion optical Temperature : 50°C
®
Injection volume : 1 μL
system (UFsensitivity), and an ultra-fast 4 5 TIC (Positive) Observed analysis column pressure : 14 MPa
scanning speed (UFscanning), enables ultra-fast TIC (Negative) × 5
analyses that produce peak widths in just a few
m/z 355 (Positive) Peaks
seconds and highly reliable data. 1: Yohimbine, [M+H] + m/z 355
m/z 250 (Positive)
2: Alprenolol, [M+H] + m/z 250
m/z 265 (Positive) 3: Tetracaine, [M+H] + m/z 265
m/z 321 (Negative) × 50 4: Chloramphenicol, [M-H] − m/z 321
5: Furosemide, [M-H] − m/z 329
m/z 329 (Negative) × 50 6: Isopropylantipyrine, [M+H] + m/z 231
m/z 231 (Positive)
0.0 0.5 1.0 1.5 min
Ultra-High-Speed Analysis of
6 Pharmaceutical Ingredients
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High Performance Liquid Chromatograph