Page 17 - Shimadzu Nexera UC - Nexera UC Prep

Extraction

Fully automated on-line extraction and purification

Nexera UC on-line SFE–SFC is a revolutionary system that combines on-line SFE, SFC and fraction collector in a single flow
path. Target compounds are extracted from solid samples and then automatically transferred to SFC/MS so that no human
intervention is required. The Nexera UC on-line SFE–SFC system reduces sample preparation time and acquires highly
accurate data. Preparation can be carried out by connecting a fraction collector.

Maximize fractionation output and labor-savings
On-line automation of the extraction and analysis of functional components On-line extraction, separation, and fractionation are possible by connecting a fraction collector at the end of the detector.
in natural products Furthermore, scale-up from analytical to preparative scale increases the volume of fractions. The Nexera UC Prep SFE unit
(maximum flow rate: 150 mL/min) with a preparative column enables high-volume extraction. On-line SFE-SFC can
Target compounds are extracted from solid samples and then automatically transferred to SFC so that no human intervention
automatically run the extraction, purification and fractionation continuously day or night. The target compounds are
is required. In the case of tomato paste, after preparing for analysis in as little as 5 minutes, extraction, separation, and
recovered in high concentrations in an organic solvent, saving time during post-run processing after preparative tasks are
detection of Lycopene are automatically carried out. In addition to a shorter sample preparation time, Nexera UC can automate
complete.
the extraction, analysis, and fractionation process with the addition of a fraction collector.
mAU
Typical sample preparation … Requires 60 minutes of sample preparation
60 min Lycopene

Repeated
dissolution
Add solvent Stir Liquid separation Collection of Evaporation to
lower layer dryness 12.5 15.0 17.5 20.0 22.5 25.0 min
Chromatogram of the extract
Reanalysis
Nexera UC … As little as 5 minutes to be ready for analysis mAU
5 min
Time reducing

Add absorbent Mix Enclose in
extraction vessel 2.5 5.0 7.5 10.0 12.5 min
Reanalysis chromatogram of the recovered solution

On-line SFE–SFC technology achieves a high recovery rate Solid autosamplers for increasing loading capacity
™
After placing the extraction vessel in position on the SFE unit and starting analysis in LabSolutions (analysis software), extraction More of the target compound can be fractionated at once by loading a large volume. However, this can sometimes lead to
and purification are performed seamlessly. poor separation and broad peak shapes because of sample solvent effects. By performing fractionation with the on-line
mAU SFE-SFC, solid samples can be enclosed in an extraction vessel and extracted directly without dissolving in a solvent. A good
peak shape prevents simultaneous elution of contaminants and improves the purity of the target component.
Lycopene
mAU
β-carotene Solid injection
(SFE)

Supercritical CO2
Solvent injection
Inside of the extraction unit
11.0 12.0 13.0 14.0 15.0 16.0 17.0 min
Inside of the extraction vessel Chromatogram of the tomato paste extract 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 min
encapsulating the sample
Chromatogram of ketoprofen (5 mg) loaded
On-line SFE-SFC enables the functional component "lycopene" to be automatically extracted from tomato paste. The amount of
extracted lycopene is equivalent to the value indicated on the product packaging.

16 17