Page 6 - Shimadzu Nexera Mikros

E a s y , F a m i l i a r O p e r a t i o n

Robust and Easy-to-Use Microflow ESI Source

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Micro-ESI Sources LC-Mikros : Innovative Microflow Pump Design
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Shimadzu’s Micro-ESI LCMS source has been designed for optimal sensitivity and ease of use. An X–Y adjustable stage and viewing camera allow for easy ESI Shimadzu LC pumps are the industry standard for long life and high performance. The LC-Mikros solvent delivery
spray needle adjustment. The column oven is mounted on the LCMS to minimize the volume between the exit of the column and the ESI needle. pump has completely new direct-drive engineering to deliver micro to semi-micro flow rates ranging from 1 to 500
The Micro-ESI probe also features an ESI spray angle optimized for microflow rates, and is available for triple quadrupole mass detection mass spectrometry µL/min. UHPLC system pressure tolerance of 80 MPa (11,600 psi) allows the use of every column type.
and quadrupole time-of-flight mass spectrometry, respectively. The LC-Mikros pump is designed for microflow gradient elution, even at low percentages. In the example below,
excellent gradient stability is shown using mobile phase compositions ranging from 1% to 90%. The LC-Mikros
delivers stable retention times essential to the chromatographer.

Average Standard
Retention Time Deviation RSD Flowrate: 5 µL/min
Gly-Tyr 5.31 0.009 0.16% Column: Shim-pack MC C18 (0.3 mm I.D. × 150 mm)
Val-Tyr-Val 7.97 0.006 0.07%
Micro-ESI 8060 Micro-ESI 9030 Angiotensin II 9.32 0.015 0.16% * Obtained under our specified conditions.
(compatible with LCMS-8045/8050/8060(NX) systems) Leu enkephalin 9.71 0.011 0.11%
Met enkephalin 10.5 0.012 0.11%
Time (min) 1 11 11.1 13 13.1 25
Concentration B 1% 40% 90% 90% 1% 1%
CTO-Mikros + UF-Link : Easy, Dependable Column Installation
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Mounted to the Micro-ESI source is the CTO-Mikros column oven. Stable column temperature, essential to retention time reproducibility, is ensured and
columns up to 150 mm in length can be accommodated. To preserve the optimal source adjustment, the CTO-Mikros features an innovative snap-in column
locking system, the UF-Link. SIL-40C XR: Low-Volume Direct Injection Autosampler
When using microflow rates, even the smallest of dead volumes can cause significant diffusion and lead to loss of peak shape and sensitivity. Shimadzu’s
UF-Link allows any user to correctly install a microflow column without disrupting the ESI spray needle position. A sure-sealing adapter allows the column to lock
into the oven and make a zero-dead volume connection with the ESI needle. Any column up to 150 mm in length with standard fittings can be accommodated. The SIL-40C XR autosampler delivers you excellent accuracy and reproducibility as well as extremely low carry-over. Its
injection volume ranges from 0.1 to 50 L and your precious sample has never wasted due to the total injection
method SIL-40C XR adapted.
Existing Ferrule Seal Method Zero Dead Volume Seal It contributes to produce highly accurate results even if you had to use low injection volume (less than 1 L). In case
of micro flow rate with narrow column, optimum injection volume also gets lowered. It is also difficult to increase the
Male nut Adapter
Ferrule injection volume, especially, if you would use a sample solvent with high elution power. In this case, sample must be
diluted to weaken the sample solvents. Highly accurate micro volume injection capability of SIL-40C XR allows you to
use samples with high organic solvent contents without dilution.
System volume and gradient delay are minimized with the newly developed “programmable loop cut-off
mechanism,” which automatically diverts the sample loop from the flow line using a high-pressure valve as soon as
the sample in needle is drained. This is extremely important in minimizing microflow analysis run times.

Dead volume Sealing part Sealing part
Injection
Injection
Connection Procedure 8,000,000 R = 0.9999 Volume (µL) Repeatability (n = 6) Volume (µL) Repeatability (n = 6)
2
Area value 6,000,000 0.1 0.80% 2 5 0.08%
0.06%
0.2
0.46%
4,000,000
2,000,000 0.5 0.32% 10 0.04%
0.7 0.21% 20 0.04%
0 1 0.06% 50 0.03%
0 10 20 30 40 50
Injection volume (µL) Injection Repeatability (Actual Values)
(1) Attach the adapter to the column. Standard (2) Place the column in the UF-Link mechanism (3) Complete the connection by swinging over
threads on the adapter make it compatible inside the oven. the lever.
with a wide variety of columns.
Nexera Mikros
6 Microflow Liquid Chromatography Mass Spectrometry System 7