Page 8 - Shimadzu LCMS-2020

Diverse Ionization Methods Expand the Range LabSolutions LCMS

of Applications

Selecting the ionization method appropriate for the target Unified software for LCMS data acquisition and processing.
compound achieves superior analysis results. Single point of control for UFLC/LCMS-2020 LCMS systems.

Easily create LC and MS acquisition parameters in a single method, and quickly review results in the Data Browser window.
LCMS-2020 offers APCI and DUIS in addition to ESI. Diverse ionization methods support a wide range of applications.

Selecting the most appropriate Ionization Method

Compounds DNA B — B Functional Acid A B A
Proteins A — B groups Alcohol C B B
Peptides A — B Aldehyde C B B
Amino acids B — B Alkane — C C
Macromolecules B C B Alkyne — B B
Carbohydrates B A A Amino A A A
Triglycerides B A A Carbonyl C B B
Aromatic hydrocarbons C C C Ester B A A
Aliphatic hydrocarbons — C C Ether C B B
Properties Polar A B A Phenol B A A
Non-polar — B B
Volatile A A A A Extremely suitable
Non-volatile A — A B Suitable Manual peak integration bar Peak table
Thermostable A A A C Analysis possible with appropriate parameters
Thermolabile A — C — Inherently unsuitable MS spectrum
table

Control Target
Ion Source Options
Data Comparison Quantitative Analysis
While the water-soluble vitamins thiamine and riboflavin can be APCI but ESI does not offer adequate detection sensitivity. Mass spectrometer (MS) and photodiode array (UV) data can be Use total ion chromatograms, extracted ion chromatograms, or
detected by ESI, they are virtually undetectable by APCI. DUIS-2020 allows detection of a mixture of compounds suited to overlaid or arranged for simultaneous viewing to discover individual UV wavelength chromatograms for quantitative
Conversely, the fat-soluble vitamin calciferol can be detected by ESI or APCI, without missing any compounds. differences in sample characteristics. A wide variety of user analysis. The browser window simultaneously loads calibration
preferences is available to interrogate spectra and chromatograms. curve and sample data for rapid analysis and review of an entire
APCI-2020 MS Chromatograms batch.
Sample
injection
Automated MS Voltage Optimization
Shimadzu's sophisticated auto-tune file comprehensively establishes MS voltages. For individual compound optimization, an easy
software tool can be used.

Corona needle

MS Spectra from DUIS Measurements
DUIS-2020
ESI probe
Analytical
conditions
are
optimized
m/z m/z m/z SIM Table Method Optimization Window Method Optimization SIM Table
Corona needle Thiamine Riboflavin Calciferol Enter the target m/z Specify the conditions to search for the Result Window The optimal voltage value is
Mixed Sample of Three Water-soluble/Fat-soluble Vitamins optimal voltage set into the SIM table
1. Thiamine: m/z 265, [M] + , water-soluble vitamin The Search results of the optimal
2. Riboflavin: m/z 377, [M+H] + , water-soluble vitamin voltage value are displayed automatically
3. Calciferol: m/z 397, [M+H] + , fat-soluble vitamin Automatic analysis execution
LCMS-2020
8 Liquid Chromatograph Mass Spectrometer 9