Page 10 - Shimadzu GCMS-TQ8040 NX

Smart Operation

Creating an MRM analysis method requires determining the optimum transitions, collision energies, and other complicated Smart MRM
parameters. Smart MRM, the method creation function, creates optimized analytical methods by using the transitions data in
the “Smart Database” file. When you want to perform MRM measurements of components not contained in the database, the Automatic Method Creation
optimal transitions can be automatically searched for using the MRM Optimization Tool.
The Smart MRM technology automatically creates methods with measurement times optimized for each component based on
the Smart database. The Automatic Adjustment of Retention Time (AART) function incorporated in the system estimates
retention times with high accuracy. When creating methods for simultaneous multicomponent analysis, the complicated process
MRM Optimization Tool of configuring measurement parameters made it difficult to prepare appropriate methods. By using the Smart MRM function,
however, it is possible to automatically create methods in which data are acquired with high sensitivity only during the elution
Optimize MRM Transitions Automatically time of the target components. In addition to MRM methods, SIM methods can be created.

Determining and optimizing MRM transitions for new compounds can require significant development time. The “MRM
Optimization Tool“ automates the process by collecting product ion scan data and finding the optimum collision energy for
each transition.
Once established, the transitions are registered to one of the Shimadzu “Smart Database” files, and the MRM or Scan/MRM
methods are created using Smart MRM.

Dichlorvos Fenobucarb
1.200 1.200
1.000 1.000
0.800 0.800
Parameter Setting
109.00 > 79.00 121.00 > 77.10
0.600 0.600
185.00 > 93.00 150.00 > 121.10
185.00 > 109.00 121.00 > 103.10
0.400 0.400 Automatic Adjustment of
Compound Retention Time (AART)
0.200 0.200
(Automatic Adjustment of Retention Time)
0.000 0.000
3 6 9 12 15 18 21 24 27 30 33 36 39 42 45 3 6 9 12 15 18 21 24 27 30 33 36 39 42 45
C17 C18 C19
Originally registered
method information
1700 1735 1800 Retention index
Smart Database ™ C17 C18 C19
Customer's Identification
instrument window
Method Management Achieved by Database File
1700 1735 1800 Retention index
The Shimadzu “Smart Database” is a database file for creating the method files using “Smart MRM” function. In addition to
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compound information and transitions, retention index can be registered in the database file. Method creation can be 20 21.1 min 21.5 min Retention time
(Originally registered RT)
proceeded without calculating the retention time by analyzing the standard samples when using the Automatic Adjustment (Calculated RT) Automatic adjustment Analyte
Retention Time (AART) function. In addition to MRM information, Scan and SIM ion information, mass spectra and calibration Automatic Estimation Dwell Time Optimization
of Retention Times
curve information from the internal standard method can also be registered in the database file. This allows users to create their The AART function adjusts the retention times of target
own database easily. components based on linear retention indices (LRI) and the
retention times of n-alkanes. The AART function easily adjusts
acquisition and processing method parameters
simultaneously.

Automatic Creation
of Analysis Methods

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