Page 8 - Shimadzu C2MAP System

The Optimal Workflow Can Be Selected

The C2MAP system supports two modes: batch mode (ordinarily used), which gives priority to the pretreatment of the culture Performing only the sample pretreatment process is possible in
supernatant samples placed, and sequential mode in which pretreatment and analysis are performed alternately for each sample. batch mode (normal mode). Batch Mode (Pretreatment Only)

Batch Mode
（Normal Mode） Sequential Mode Deproteinization
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5
Pretreatment
Sample 1 Sample 2 Sample 3 of sample 1
Dilution
Deproteinization
Deproteinization
Pretreatment
of sample 1
Dilution Culture supernatant is recovered each Deproteinization
culture day, then frozen and stored.
Dilution
Pretreatment
of sample 2
Completion of a series of cultures Dilution
Deproteinization
Pretreatment
of sample 2 Analysis Deproteinization
Dilution Pretreatment of Analysis of Deproteinization
all samples all samples
Pretreatment
of sample 3
Dilution
Deproteinization Dilution

Pretreatment
of sample 3
Dilution Analysis Deproteinization

Dilution
Analysis

Measurement of Analysis Implement pretreatment Replace the samples Implement pretreatment
all pretreated Analysis of 65 samples and the MTP of 65 samples
samples

Analysis

After pretreatment, After pretreatment,
the samples are the samples are
In batch mode, sample deproteinization is given priority. In sequential mode, measurements by LC/MS/MS and stocked in the MTP. stocked in the MTP.
As a result, enzyme proteins existing in samples can be deproteinization procedures with the C2MAP-2030 are
inactivated, improving the stability of the samples. carried out concurrently, so the overall analysis time can By using two MTPs, consecutive analyses of up to 130 samples can be performed.
be shortened. Note: Rack switching is required.

C2MAP System
8 9
Cell Culture Media Analysis Platform