Page 11 - Shimadzu C2MAP System

Supports a Wide Range of Measurement Compounds C2MAP TRENDS

and Culture Supernatant Samples

The analysis method stored in the control software allows the simultaneous analysis of the following 95 components plus For results obtained via LC/MS/MS, temporal changes in each component can be graphed by the dedicated viewer software. Analysts
2-Isopropylmalic acid. can monitor variations in metabolites secreted from cells and culture media components during cultivation, as well as display graphs
of component comparisons with samples from different culture series. As a result, the consumption and depletion of culture media
components, and changes in the amounts of metabolic components secreted from cells, can be observed, thereby providing useful
Internal Standard Amino Acid and Derivatives Vitamins insights about the optimal culture conditions and assessments of cellular status.
2-Isopropylmalic acid 2-Aminoadipic acid 4-Aminobenzoic acid
4-Aminobutyric acid Ascorbic acid
4-Hydroxyproline Ascorbic acid 2-phosphate Temporal Changes in Measured Components
Sugars 5-Glutamylcysteine Biotin
Gluconic acid
5-Oxoproline Choline
Glucosamine Alanine Cyanocobalamin
Hexose (Glucose) Alanyl-glutamine Ergocalciferol
Sucrose
Arginine Folic acid
Threonic acid Asparagine Folinic acid
Aspartic acid Lipoic acid
Citrulline Niacinamide
Nucleic Acid Associated Compounds Nicotinic acid
Adenine Cystathionine Pantothenic acid
Cysteine
Adenosine Pyridoxal
Adenosine monophosphate Cystine Pyridoxine
Cytidine Glutamic acid Riboflavin
Glutamine
Cytidine monophosphate Tocopherol acetate
Deoxycytidine Glutathione
Guanine Glycine
Guanosine Glycyl-glutamine Others
Guanosine monophosphate Histidine
Hypoxanthine Isoleucine 2-Aminoethanol
Inosine Kynurenine 2-Ketoisovaleric acid
Leucine 3-Methyl-2-oxovaleric acid
Thymidine
Thymine Lysine 4-Hydroxyphenyllactic acid
Uracil Methionine Citric acid
Methionine sulfoxide Ethylenediamine
Uric acid Fumaric acid
Uridine N-Acetylaspartic acid Measured-component Comparisons among Different Culture Series
Xanthine N-Acetylcysteine Glyceric acid
Xanthosine Ornithine Histamine
Oxidized glutathione Isocitric acid
Phenylalanine Lactic acid
Antibiotics Pipecolic acid Malic acid
Penicillin G Proline O-Phosphoethanolamine
Serine Putrescine
Threonine Pyruvic acid
Tryptophan Succinic acid
Tyrosine
Valine

It is confirmed that the C2MAP system can accommodate the following culture media and culture media additives.

Cell Type CHO cells iPS/ES cells T cells Mesenchymal Cell Type
BalanCD CHO AK03N X-VIVO 10 MSCBM ™
®
™
Culture Media 1×CD CHO Essential-8 ™ X-VIVO ™ 15 MesenPRO ™
EX-CELL ® CHO mTeSR™1/TeSR -E8 ™ TexMACS ™ Stempro ®
™
Additives Fetal Bovine Serum（100% v/v）

C2MAP System
10 11
Cell Culture Media Analysis Platform