Page 5 - Shimadzu C2MAP System

Example of Operator Labor
Time Comparison
Features of the C2MAP System *Labor Time for 65 Samples

Total

CHO cells, iPS cells Remove floating cells/dead
ES cells, T cells, cells by filtering or centrifugation
mesenchymal stem cells (manual pretreatment is required 475 min 70 min
beforehand).
Automated Process from Pretreatment to Culture Solution Conventional C2MAP System
Measurement for the Culture Supernatant Analysis *An additional post-run analysis process is
necessary. The post-run analysis is not
1 •The automated process allows analysis to be performed at night and on included in the labor time calculation because
the process is common to both the
non-working days.
conventional method and the C2MAP system.
•The measurement workflow can be selected to match the actual culture.
•Seamless analysis and management from pretreatment to LC/MS/MS
measurement can be achieved. Performed by the C2MAP-2030 Pretreatment-related Process
•Pretreated samples are stocked on a microplate automatically to enable Cell Culture Media Analysis Platform
re-measurement with ease.
Addition of internal standard
Denaturation of proteins by adding 155 min 35 min
organic solvents
Stirring Conventional C2MAP System
Supports a Wide Range of Measurement Suction filtration

Compounds and Culture Supernatant Sample delivery to the HPLC autosampler *For the C2MAP system, the time required
for preparation of C2MAP-2030 is shown
2 Samples because it performs pretreatment process
automatically.
*Time to remove floating cells and dead cells
•A total of 95 components, including major basal culture media components for
animal cells and secreted metabolites, can be simultaneously analyzed at high speed. Performed by the SIL-30AC are not included.
•Applicable to a wide range of cell culture media
(iPS cells, ES cells, mesenchymal stem cells, T cells, and CHO cells) Automatic sample dilution
Dispensing to the MTP

Visualization of Component Sample Registration Process
3 Variations in Culture Media Performed by the LCMS-8060/8050
Prepared dedicated control software
•Temporal changes in the components can be displayed as trend graphs.
•The results under multiple experimental conditions can be overlaid in the display, Measurement by LC/MS/MS 20 min 15 min
enabling comparative analysis. Simultaneous analysis of up to 95
components using a cell culture
Conventional C2MAP System
profiling method
*LC/MS/MS analytical time is not included.

Graph Making Process
4 •By installing the automatic pretreatment unit separately, pretreatment and LC/MS/MS Visualization of temporal changes
Supports Flexible System Configurations
Performed by the C2MAP TRENDS
measurements can be performed in different rooms. in each component 300 min 20 min

Conventional C2MAP System
*Time comparison for graph making by C2MAP
TRENDS and a spreadsheet is shown.

C2MAP System
4 5
Cell Culture Media Analysis Platform