Page 2 - Shimadzu C2MAP System

LC/MS/MS Cell Culture Media Analysis Platform Provides High-Quality Measurement Data with Ease

Capable of Obtaining Temporal Change Profiles

for Up to 95 Culture Supernatant Components

Material
Production Cell Therapy Culture Media

・ Development
• Optimization of ・ Marker Search
of Culture Media
Culture Process for Quality Evaluation
・ Optimization
・ Evaluation
of Media Composition
of Scale-up

Antibiotics 1 Internal Standard 1
Others
16 Amino acids For component variation
Automatic pretreatment of Sugars 5 and Derivatives by C2MAP TRENDS Graph Display
™
culture supernatant samples Vitamins 38
17 C2MAP-2030 LCMS-8060/8050
Nucleic acid
associated compounds Cell Culture Media Analysis Platform Optimized cell culture profiling LC/MS/MS
18
Up to 65 samples can be pretreated method package enables high-speed analysis
and delivered to the HPLC of 95 components.
Basal medium component / Secretory metabolite automatically.
Simultaneous LC/MS/MS analysis
of up to 95 components

Visualization of Temporal Change for Component Variations in Culture Media Modules Required for Automated Pretreatment Can Be Operated Independently
of LC/MS/MS
The C2MAP System can be automated the process from pretreatment to measurement for culture supernatant samples and
™
display temporal changes in the components as graphs.
With the optimized LC/MS/MS simultaneous analysis method, the system can be used for the optimization of culture
conditions in animal cell cultures by monitoring the consumption and depletion of media components during culturing, as
well as the variation in metabolites secreted from cells. The LC/MS/MS can be installed in a different room, allowing to be shared for other
analyses.
If culture samples cannot be taken out of the culture room, sample pretreatment can be
performed in the culture room and measured by LC/MS/MS in another room.
Separating the various components saves laboratory space.