Page 8 - Shimadzu BioSpec-nano

Stress-free Nucleic Acid & Labeled Nucleic Acid Quantitation Outstanding Analysis Range & Measurement Reproducibility

Simple Nucleic Acid Quantitation Mode Protein Quantitation Mode

For the quantitation of RNA, dsDNA, ssDNA, The protein concentration is
and OligoDNA. calculated from the molar
The nucleic acid concentration and OD ratio absorption coefficient.
(OD260/280 and OD260/230) are calculated.

[Simple Nucleic Acid Quant. - Detailed View Mode]
[Measurement sample: Purified dsDNA in TE buffer] [Protein Quantitation (OD280 Method) - Detailed View Mode]
(Measurement Sample: BSA)

[Analysis Setting Windows - Simple Nucleic Acid Quant.] [Analysis Selection Window - Protein Quantitation (OD280 Method)]

Labeled Nucleic Acid Quantitation Mode Labeled Protein Quantitation Mode
For the quantitation of nucleic Cy3, Cy5, Alexa Fluor 546, and
acids (RNA, dsDNA, ssDNA, Alexa Fluor 647 Note 5) are used for
OligoDNA) labeled by Cy3, Cy5, quantitation of labeled proteins.
Alexa Fluor 546, and Alexa Fluor The protein concentration, label
647 Note5) . concentration, and labeling rate are
The nucleic acid concentration, calculated.
label concentration, nucleotide
concentration, and labeling ratio
are all calculated.

[Labeled Nucleic Acid Quant. - Detailed View Mode]
[Measurement Sample: OligoDNA labeled by Cy3 in TE buffer]
[Labeled Protein Quantitation - Detailed View Mode]
(Measurement Sample: Alexa Fluor 546-IgG)

Note 5) Four dyes are pre-registered. An additional 8
[Analysis Setting Windows - Labeled Nucleic Acid Quantitation] new labels can be registered. [Analysis Selection Window - Labeled Protein Quantitation]

BioSpec-nano
8 Spectrophotometer for Life Science 9