Page 6 - Shimadzu BioSpec-nano

Quick & Simple Operation Outstanding Measurement Accuracy & Reproducibility

Basic operations can be conveniently performed by clicking icons in the software or function keys on the instrument itself. [Secure Analysis / High Measurement Accuracy]
Organizing data is also easy even when there many samples.
As little as 1 – 2 µL of undiluted sample can be measured as is.
High reproducibility is obtained even when measuring low concentration samples.
Start High correlation is achieved with the measurement values provided with the double beam spectrophotometer.

Example of 10 Successive Measurements of the Same Sample (Double Stranded DNA)

Pathlength: 0.2 mm (Example of analysis at 1 µL) Pathlength: 0.7 mm (Example of analysis at 2 µL)
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40 CV 0.84 % with OD Value 40
(Concentration: 2,000 ng/µL) 5
Measured OD260 20 Measured OD260

CV 1.4 % with OD Value 1.5
(Concentration: 75 ng/µL)
0 0
0 20 40 0 5
Correct OD260 Correct OD260
Sample : Diluted purified double stranded DNA
Display of Measurement Results Detail / List Buffer : TE
Instrument : Shimadzu double beam spectrophotometer Measurement using 1 mm cell and 0.1 mm cell.
Toggles between the Detail and List view modes. Example of 10 Successive Measurements of the Same Sample (BSA = Bovine Serum Albumin)

Detail Displays the analysis results and spectrum of the sample currently selected.
List Displays the analysis results of all samples in a table format. Pathlength: 0.2 mm (Example of analysis at 3 µL) Pathlength: 0.7 mm (Example of analysis at 4 µL)
Converts analysis results to PDF files, and displays them using Adobe Reader.
CV 1.05 % with OD Value 43 8
(Concentration: 65,000 µg/mL)
CV 0.98 % with OD Value 4
40 6 (Concentration: 6,060 µg/mL)
Measured OD280 20 Measured OD280 4

0 2
0 20 40 0
0 2 4 6 8
Correct OD280 Correct OD280
Saves Analysis Results
Sample : BSA
Saves analysis results to CSV or PDF files. CSV files can be edited in Microsoft Excel or other spreadsheet software. Buffer : Distilled water
Instrument : Shimadzu double beam spectrophotometer Measurement using 1 mm cell and 0.1 mm cell.

Analysis Data judgment
Display PDF Save CSV/PDF
If a droplet is not properly formed for some reason, such as because of, the depositing of insufficient volume, or if the sample contains
bubbles, a larger OD800 value is generated. If the measured value is outside the range of the set OD800 value, is displayed,
allowing confirmation of the measurement validity.
(Note: This applies to sample solutions that do not exhibit absorption at 800 nm.)

BioSpec-nano
6 Spectrophotometer for Life Science 7