Page 11 - Shimadzu BioSpec-nano

Photometric Measurement BioSpec-nano Specifications

Photometric Measurement Mode

OD values can be displayed for up to 8 freely selected Hardware Specifications
wavelengths. Item Specification Item Specification
Measuring wavelength range 220 to 800 nm Detector Photo diode array
Spectrum bandwidth 3 nm Auto wiping function Provided
Wavelength accuracy ±1 nm Sample mount function Auto
Pathlength 0.2 mm, 0.7 mm (manual selection) Spectrum measuring time 3 sec
Photometric range 0 to 1.5 Abs Quantitation range* Pathlength 0.2 mm, 1 to 75 OD 50 to 3,700 ng / µL
Photometric value unit OD (Optical Density), absorbance converted with 10 mm pathlength (OD, dsDNA concentration) Pathlength 0.7 mm, 0.3 to 21 OD 15 to 1,000 ng / µL
Option cell Available (pathlength: 5 mm, sample volume: 2 mL) Optional 5 mm pathlength cell, 0.04 to 3 OD
Pathlength lever switched to Option (5 mm) position Power requirements 2 to 150 ng / µL
Sample volume 1 µL min. (pathlength: 0.2 mm) AC 100 V / 120 V / 220 V / 230 V / 240 V, 50 / 60 Hz, 40 VA
2 µL min. (pathlength: 0.7 mm) Ambient temperature, humidity 18 to 28°C, 40 to 80% (without dew condensation)
Light source Xenon flash lamp Dimensions & Weight Width 210 mm x Depth 214 mm x Height 417 mm
Monochrometer Holographic grating 7 kg
*Quantitation range is a range which can secure the accuracy (±10% as a guide)
specified by us.

Software Specifications
Item Specification Item Specification
Analysis mode Simple nucleic acid quantitation, labeled nucleic acid quantitation, Analysis results display Detailed view (displays the focused sample analysis results and spectrum)
protein quantitation, labeled protein quantitation, photometric measurement List view (displays analysis results of all samples)
[Photometric Measurement - Detailed View Mode] Simple nucleic acid quantitation Nucleic acid concentration (RNA, dsDNA, ssDNA, OligoDNA) calculation, Analysis data judgment OD ratio (OD260 / 280, OD260 / 230), OD 800 judgment
(Measurement Sample: BSA)
OD ratio (OD260 / 280, OD260 / 230) calculation PDF output Analysis results (detailed view, list view) saved in PDF file
Labeled nucleic acid quantitation Nucleic acid concentration (RNA, dsDNA, ssDNA, OligoDNA), CSV output Analysis results (detailed view, list view), spectra data saved in tab delimited text
nucleotide concentration calculation, Label concentration, labeling ratio calculation, User management Multiple user or single user mode selected at the time of installation
OD ratio (OD260 / 280) calculation (Multiple user mode) User management: User registration, edit, deletion
Protein quantitation Protein concentration (M, µg/mL), label concentration, labeling ratio Login: User name, password entry
[Analysis Selection Window - Photometric Measurement] Photometric measurement OD display for 8 wavelengths maximum Data saved in respective folders
Label management Label registration (up to 8 new labels), edit, deletion
Default labels (Cy 3, Cy 5, Alexa Fluor 546, Alexa Fluor 647)

Option Cell (Pathlength: 5 mm)

The 5 mm pathlength cell and 5 mm cell adapter are PC Requirements for BioSpec-nano * A separate PC is required.
required separately. Item Specification
The optional 5 mm pathlength cell and its adapter are OS Windows 7 Professional 32/64bit edition
placed over the target for use as shown below. Windows 10 Pro 64bit edition
CPU 1.6 GHz or higher processor
System memory 512 MB or larger (1 GB or larger when using Windows Vista)
Hard disk capacity
40 GB or larger
Measured OD260 Display resolution 1024 × 768 pixels or above H: 417 mm
USB 2.0 port
1 port or more (for connecting the instrument),
Printer 2 ports or more when using a USB printer
Printer compatible with the system used
Disk drive DVD-ROM or CD-ROM drive

Consumables
Correct OD260
P/N Part Name
Example of analysis at 2 mL* 206-25901 Wiping paper (highly absorbent wiper, 100 sheets/set)
W: 210 mm D: 214 mm
* All of the analysis example data are provided for reference only.
1 OD corresponds to 50 ng/µL dsDNA.
Sample: Purified dsDNA
Buffer: TE
5 mm pathlength cell 5 mm cell adapter Ten measurements were performed at each point.
(sample volume: 2 mL) (P/N: 206-26513)
(P/N: 208-92258)

BioSpec-nano
10 Spectrophotometer for Life Science 11