Page 5 - Shimadzu BioSpec-nano

Drop-and-Start Analysis [1 µL / 2 µL samples can be measured.] Note2) Automatic Optical Pathlength Setting & Automatic Wiping

Just drop the sample onto the target and click the button. That's all there is to analysis. The instrument will perform everything else for From setting the optical pathlength to measurement to wiping away the sample, it’s all automatic.
you – sample mounting, measurement and wiping. Liquid-contact parts no longer need to be wiped with a cloth. Tedious operations, like raising and lowering the arm to set the pathlength and wiping off the sample with a piece of cloth, are no longer
required. There is no need to worry about whether the sample has been thoroughly wiped away, or whether the measurement site might
become scratched.
1. Drop the sample

Sample volume required for
measurements are :
1 µL for pathlength 0.2 mm
2 µL for pathlength 0.7 mm

Depositing sample
2. Outstanding analysis range &
measurement reproducibility

The optimum pathlength for the
sample concentration can be selected
Upper window
using the instrument lever.
Target
Lowering window and measurement Sample wiping and raising window

3. Start measurement Automatic sample wiping
allows the analyst to
deposit the next
Click the [start] button to start sample Low Carryover Achieved with Automatic Wiping Feature
measurement sample while
measurement.
the current sample is being
You will find using the [start] button on
measured without having The automatic wiping function of the BioSpec-nano greatly measurements. In order to determine the level of carryover from
the instrument handy when performing to put down the pipette. minimizes sample carryover from one measurement to the next. the measured DNA sample, measurement of the next blank was
analyses with gloves on. Button in software Below are the results of alternate measurement of a double conducted without replacing the wiper during the analyses. The
window Measurement is easy even
when there are many stranded DNA sample and a blank (TE buffer), each measured 60 results indicated that carryover Note 4) was extremely low, averaging a
samples. times with automatic one-time wiping Note 3) between mere 0.13%.
Instrument [start] button
Note 3) Automatic wiping following measurement can be set to a maximum of 5 times. Normally, wiping just once is sufficient to obtain good
results, however, wiping 3 times is recommended for samples having high viscosity, such as protein samples.
4. Check the analysis results Note 4) Carryover (%) = (calculated DNA concentration when measuring TE buffer / concentration of DNA measured immediately before) x 100.

Analysis results are automatically
displayed after measurement ends. Measurement Values from 60 Alternate Measurements Each of Double Stranded DNA Sample and Blank (TE Buffer)
A series of samples can be analyzed
while confirming spectra in the
Detailed View Mode. 15

OD Value 10

5
0
[Simple Nucleic Acid Quant. - Detailed View Mode]
[Measurement Sample: Purified dsDNA in Tris-EDTA (TE) buffer] 0 20 40 60 80 100 120
Number of Measurements
Note 2) When measuring substances like proteins that do not easily form droplets, deposit more than 1 – 2 µL of sample.
The results can be checked using the analysis data judgment feature. (See P. 7)

BioSpec-nano
4 Spectrophotometer for Life Science 5