Page 124 - Shimadu Consumables Catalog

Reversed-phase Columns

Shim-pack STR ODS-M

Bonded Phase Octadecyl Group
Particle Size 5 μm
Particle Shape Perfectly spherical porous silica gel
Pore Size 10 nm
2
Specific Surface 350 m /g
End-Cap Yes
Carbon content 15 %
USP Code L1

AMP, ADP, ATP
1. Adenosine 5’-Monophosphate
2. Adenosine 5’-Diphosphate
3. Adenosine 5’-Triphosphate
Column : STR ODS-M, 150 mm x 4.6 mm, I.D. (226-80100-01)
Mobile Phase : 30 mM 2-Diethylaminoethanol is contained
20 mM Citric Acid Aqueous Solution
Flow Rate : 0.8 mL/min
Col. Temp. : 40 °C
Detection : UV-260 nm

Caffeine and Catechin
1. Epigallocatechin 4. Epigallocatechin Gallate
2. Catechin 5. Epicatechin
3. Caffeine 6. Epicatechin Gallate
Column : STR ODS-M, 150 mm x 4.6 mm, I.D. (226-80100-01)
Mobile Phase : A: 10 mM-Phosphate Buffer (pH2.6)
B: CH3CN
7 % B g 7 % B (30 min) 12 % B (30.01 min) g 12 % B
(45 min) g 50 % B (45.01 min ) g 50 % B (50 min) g 7
% B (50.01 min) g 7 % B (80 min)
Flow Rate : 1.0 mL/min
Col. Temp. : 45 °C
Detection : UV-280 nm (0.64 AUFS)

Particle Size Length (mm)
Column I.D (mm)
(μm) 10 50 150 250
4.0 226-80103-01 226-80104-01 226-80098-01 226-80099-01
STR ODS-M 5 4.6 - - 226-80100-01 226-80101-01
6.0 - - 226-80102-01 -

Pg A-120