Page 7 - QAD-TOF syetem

Surprisingly simple

Positive/negative polarity switching opens possibilities for Effortless operation and reliable results
new applications

Easy to switch between CID and OAD
Shimadzu's breakthrough innovation challenges the common belief that TOF instruments cannot undergo
efficient polarity switching. With the capability to achieve low-ppm mass measurement accuracies in short CID is also possible with the OAD RADICAL SOURCE I
switching intervals, possibilities are now open to enhance the workflow with greater confidence and ease.
installed. No special software is required, and
LabSolutions LCMS control and analysis software for
™
LCMS-9050 can easily switch between OAD and CID for
Achieving both polarity switching and analysis. Acquisition of a large amount of structural
Mass accuracy information by OAD and CID can lead to new discoveries
in lipids and other organic compounds.
Measure Measure Measure Measure Measure The LCMS-9050 features a proprietary
Positive Negative Positive Negative Positive
ions ions ions ions ions high-precision electrical circuit and the
UFstabilization algorithm (patent
™
Switch Switch Switch Switch [M + H] +
polarity polarity polarity polarity Reliable structure estimation with
pending) that make polarity switching 687.5537
Illustration of Switching between both fast and stable. high mass accuracy
Positive and Negative Ion Modes
∆m/z < 3 ppm
The high mass accuracy achieved with the LCMS-9050
n-9
remains unchanged even with OAD. High-quality MS/MS 591.4231
O CH3
Increasing workflow efficiency with polarity switching data within ±3 ppm enables reliable structure estimation O O CH3
O
of compounds, including determination of the position O CH3 TG 18:1 (n-9)_16:0_4:0
O
Analysis that was previously performed twice in positive ion mode and negative ion mode can now be of carbon-carbon double bonds.
performed in one analysis, improving the analysis throughput. In addition, positive and negative ions can be
560 580 600 620 640 660 680 m/z
detected in the pretreated sample under the same conditions without any time lag, improving the reliability
of the analytical data.
100 25.0
[M + H] +
90 782.5688 22.5
255.2325 C16:0
80 20.0
×20
70 17.5 303.2326 C20:4
688.4541
60 n-9 15.0
648.4224 728.4868
% 50 n-12 n-6 % 12.5
40 608.3912 10.0
184.0732 n-15
30 7.5
20 Fragment ions derived from 5.0
the polar group
10 2.5
0 0.0
200 300 400 500 600 700 800 m/z 200 300 400 500 600 700 800 m/z
Positive Ion Mode Measurement (OAD) Negative Ion Mode Measurement (CID)

PC (36:4) and other lipids were detected in
O O
+ O P O O mouse liver samples in both positive ion and
N
O H O negative ion modes. Taking measurements in
O both positive ion and negative ion modes
enables more accurate structural estimates of
compounds.

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