Page 17 - Application Notebook - Hand Sanitizer Analysis

Application No. A626
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„ Pass/Fail Judgment Using LabSolutions UV-Vis

The provisions for “Other impurities (absorbance)” of anhydrous
ethanol specify that the absorbances at 240 nm, between 250
and 260 nm, and between 270 and 340 nm are not more than
0.40, 0.30, and 0.10, respectively. However, reading the
absorbances of all the measured samples is time-consuming
work and judgments must be made carefully, as human error is
also possible. The time required for this work can be reduced by
the spectral evaluation function of LabSolutions UV-Vis.
The spectral evaluation function features 8 pass/fail criteria
and 33 standard evaluation methods, including point pick,
maximum value, minimum value, peak, valley, area, statistics,
and cutoff, as well as a pass/fail judgment function. Here, a
pass/fail judgment for anhydrous ethanol was made using
the pass/fail judgment function.
Fig. 4 Detailed Settings Screen for
[Maximum Value – Single Point] Evaluation
„ Setting of Pass/Fail Judgment Using
LabSolutions UV-Vis „ Results of Pass/Fail Judgment
Although there are three judgment conditions by absorbance, Fig. 5 shows the results of the pass/fail judgments for the
in analysis of anhydrous ethanol, judgments are made by using anhydrous ethanol shown in Fig. 2 and a simulated rejected
[Point Pick – Single Point] and [Maximum Value – Single Point]. sample. The absorbance values of the anhydrous ethanol are
[Point Pick – Single Point] reads the absorbance of a fixed within the pass range for all three wavelength conditions. On
wavelength, and [Maximum Value – Single Point] can read the the other hand, in case of failure, the result can be
maximum value in a predetermined wavelength range. understood at a glance, as the evaluation line is colored red.
First, absorbance of NMT 0.40 (“not more than 0.40”) at Fig. 6 shows an enlarged view of the evaluation table.
240 nm is set. Fig. 3 shows the Detailed Settings screen for In pass/fail judgments, the data are added to the evaluation
evaluation of [Point pick – Single point]. The setting is made table automatically after spectrum acquisition. Therefore, the
from this screen. The wavelength is set at 240 nm, and the judgment can be completed simply by measuring the sample.
pass/fail judgment criterion is set at NMT 0.40.
- : Anhydrous ethanol
- : Simulated rejected sample

Fig. 5 Results of Pass/Fail Judgment
Fig. 3 Detailed Settings Screen for
[Point Pick – Single Point] Evaluation
„ Conclusion
Next, the remaining conditions are set. Fig. 4 shows the In this experiment, measurements of anhydrous ethanol were
Detailed Settings screen for evaluation of [Maximum Value – conducted in accordance with the Japanese Pharmacopoeia,
Single Point]. The wavelength range is designated, and the European Pharmacopoeia, and United States Pharmacopeia
maximum value of absorbance in that range is read. using a UV-1900i, and a pass/fail judgment was made using the
Here, two conditions are set. The judgment criteria of NMT spectral evaluation function of the LabSolutions UV-Vis
0.30 is set for 250-260 nm, and NMT 0.10 is set for 270-340 nm. software. Analysis time, including judgment work, can be
substantially reduced by using the spectral evaluation function.

Fig. 6 Enlargement of Evaluation Table

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First Edition: Jul. 2020
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