Page 91 - Application Handbook - Liquid Chromatography

SCA_280_081

High Performance Liquid Chromatography

Analysis of amoxicillin and cefotaxime

No. SCA_190_022

Alban Huteau, Shimadzu France SAS.

Introduction Analytical Conditions:

Amoxicillin, is an antibiotic useful for the treatment System configuration:
of a number of bacterial infections. It is the first line
treatment for middle ear infections. It may also be A Shimadzu Nexera X2 UHPLC system was used
used for strep throat, pneumonia, skin infections, consisting of two quaternary solvent pumps (LC-
and urinary tract infections among others. It is 30AD) with two 5 channel degassers (DGU-
taken by mouth. 20A5R), an autosampler (SIL-30AC), and a column
Cefotaxime is a third-generation cephalosporin anti- oven (CTO-20AC). The system was also equipped
biotic. Like other third-generation cephalosporins, with an SPD-M30A photo diode array detector.
cefotaxime is a broad-spectrum antibiotic with
activity against numerous Gram-positive and Analytical Method:
Gram-negative bacteria. Developed by Hoechst-
Roussel Pharmaceuticals in the late 1980s, it was Column: Kinetex C18, 100 x 2.1 mm 2.6 µm
among the first third-generation ("extended Mobile Phase: A: H2O + 2 mM CH3COONH4;
spectrum") cephalosporins and the first of its class B: MeOH + 0.1% FA
to become available in the United States. It is on Gradient: 10 % B (0-0.5 min); 95 % B
the World Health Organization's List of Essential (4-6.5 min); 10 % B (6.8-10 min)
Medicines, the most important medications needed Flow rate: 0.4 mL/min
in a basic health system. Temperature: 30 °C
Injection Volume: 0.5 µL
Detector: SPD-M30A, standard cell,
sampling rate 12.5 Hz, time
Chromatogram constant 0.160 sec, slit width 8 nm

Conclusion /Result

A fast, selective, and sensitive method for the Very good results were also obtained in deviation
analysis of amoxicillin and cefotaxime was and accuracy and for the calibration curve a
developed. correlation coefficient from >0.999 was achieved.