Page 3 - 4_TOC special applications
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Sum parameter – Total Organic Carbon
TOC- determination in algal biomass –
suspension method
SCA-130-401
■Innovative methods
To determine the biomass in the
photobioreactor, a TOC analyzer was used.
The carbon content of the ‘algal soup’ is
directly proportional to the biomass.
The excessive global CO 2 emissions from the
burning of fossil fuels (for instance in power
plants) causes the search for climate-friendly
uses of carbon dioxide.
One of the approaches for environmentally
sound recycling is to convert the emitted CO 2
into biomass using photobioreactors.
■ TOC Measurement method
The CO 2 gas is introduced into the Depending on the type of algae used in the
photobioreactor in order to be used for the reactor, either the difference method or the
growth of algae. The biomass, or algae, can direct method (NPOC) is suitable. In both
be used in many different application areas: cases, one should test which method will
in the cosmetics industry, the construction most accurately detect each particular type of
industry, and the food segment, in agriculture algae. This can be compared with the results
as fertilizer or for energy utilization. of the reference method.
Information on the analysis:
■ Test methods for implementation calibration of the TC/NPOC and the IC
The efficiency of the photobiorectors and the parameters via the automated dilution
yield of growth are continuously monitored. function
To this end, various methods are available, sample is generally measured undiluted
including the determination of dry mass injection volume: 90 µL
(gravimetric) or the photometric determination at least 3 to 5 injections for statistical
of chlorophyll (by absorption). These methods confidence
either require a high expenditure in terms of rinse several times, depending on the
time and personnel, or they are nonspecific sample
and inaccurate.
