Page 59 - Pharmaceutical Solution for Pharma Analysis

3 Comparison of GC-FID and GC-MS Chromatograms
After using a GC-FID system to acquire data from a Class 2 standard solution A, data were acquired using a GC-MS system with the
same linear velocity condition (35 cm/sec). Then the resulting chromatograms were compared. The comparison shows that the shift
in retention times between FID and MS data was 0.02 minutes (1.2 seconds) for acetonitrile, which elutes early, and for cumene,
which elutes late. In addition, both chromatograms were similar, with approximately the same separation patterns.

FID

Acetonitrile Cumene
(4.05 min) (29.13 min)

MS

Qualitative analysis
based on the mass
spectrum displayed
Acetonitrile Cumene
(4.07 min) (29.15 min)

The GC-MS data showed a peak at roughly the same retention time as for the unknown peak detected by the GC-FID system. This
peak can be identified easily by qualitative analysis, such as by displaying the mass spectrum and using an MS spectral library to
search for a similar peak pattern.

4 Summary
If a HS-GC-MS system is used for qualitative analysis in testing for residual solvents in pharmaceuticals, it is important that the
chromatogram pattern obtained is similar to the chromatogram obtained from HS-GC-FID analysis. The constant linear velocity
control mode for the AFC unit included in Shimadzu GC-MS systems can be used in combination with a Shimadzu HS-20 headspace
sampler. Even if the different-sized columns are used for HS-GC-FID and HS-GC-MS analysis, chromatograms with similar retention
times and separation patterns can be obtained easily by using columns with the same phase ratio and by specifying the same linear
velocity setting, which means GC-MS data can easily be used for qualitative analysis.

For Research Use Only. Not for use in diagnostic procedures.
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