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Nexera UC

Supercritical Fluid Chromatography System for Chiral Screening

When considering analytical conditions for the analysis of chiral compounds, various combinations of Combining the Nexera UC Chiral Screening System with Diacel columns
analytical columns and mobile phases need to be evaluated through trial and error, resulting in considerable
Here we present the results of screening 36 SFC analysis Scouting conditions
time and labour taken up by method development.
conditions resulting from combinations of 12 Daicel columns
This process can be streamlined and sped up signi cantly with the use of Shimadzu's Nexera UC Chiral
and 3 modi ers using the Nexera UC Chiral Screening System. Chiral columns for SFC
Screening System for screening analytical conditions, in combination with Daicel's CHIRALPAK and CHIRALCEL Because the system can switch automatically between
series chiral separation columns. modi ers and columns during analysis, the analysis can be (1) CHIRALPAK IA-3 (7) CHIRALPAK AD-3
(8) CHIRALPAK AS-3
(2) CHIRALPAK IB-3
carried out continuously night or day, allowing for speedier (3) CHIRALPAK IC-3 (9) CHIRALPAK AY-3
(4) CHIRALPAK ID-3 (10) CHIRALCEL OD-3
evaluation of analysis conditions. The example shown on this
(5) CHIRALPAK IE-3 (11) CHIRALCEL OJ-3
page was completed in one night. (6) CHIRALPAK IF-3 (12) CHIRALCEL OZ-3
Data can be collected by simply setting the samples and All columns have I.D. 3.0 mm, length 100 mm, particle size 3 m
mobile phases and initiating analysis, resulting in a signi cant
Modi ers
reduction in labor. The user is free to carry out other tasks
while analysis is in progress, increasing the overall productivity (1) Methanol
(2) Ethanol
of the lab. (3) Acetonitrile/Ethanol = 75/25 (V/V)
In addition, Shimadzu's LabSolutions HPLC workstation data For all analyses CO2/modi er = 8/2 (V/V)
browser assists in fast and reliable data processing after
Automatic analysis for all
evaluation of chiral analysis conditions. A large amount of
36 SFC chiral screening conditions
scouting data can be viewed at once, along with
chromatogram data such as retention times, area values,
separation values, and symmetry factors.

Modi er : MeOH Modi er : ACN/EtOH = 75/25 (v/v)
IA IB IC ID IA IB IC ID

Evaluating analytical conditions with the Nexera UC Chiral Screening System
IE IF AD AS IE IF AD AS
When using the system for supercritical uid chromatography (SFC) analysis of chiral compounds, with the mobile phase being
a supercritical uid with high diffusivity, it is possible to carry out analysis in 1/3 to 1/5 the time compared to a conventional
AY OD OJ OZ AY OD OJ OZ
HPLC system. The Nexera UC Chiral Screening System not only allows for SFC analysis, but can also automatically switch
between up to 12 columns, 4 modi ers, and blends of those modi ers during analysis, greatly reducing the overall work ow.
With the dedicated software, "Method Scouting
Modi er : EtOH mAU
Solution", the columns and modi ers to be used can
be managed in a database, and various analytical IA IB IC ID CHIRALCEL OZ-3/SFC
MeOH
600
conditions can be applied by simply selecting them
from the graphical user interface (GUI).
IE IF AD AS 400 Resolution: 7.97
It supports evaluation of analytical conditions in
multiple ways: calculating modi er and sample 200
amounts required, prevention of degradation through AY OD OJ OZ
column rinsing and changeover of enclosed uids 0
post-analysis, and prediction of analysis completion 0.0 1.0 2.0 3.0 4.0 min
times. Screening Results

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