Page 78 - Clinical-Medical Solution for Clinical Chemistry

Application No.C98
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n Peak Determination Using Multiple Reference Ions
When using multiple reference ions to conduct high- Reference ion mode can be
accuracy identification, the process of selecting and selected from among:
making the associated entries becomes complicated. (1) (2) (3) ɾAbsolute
ɾRelative
As of Labsolutions Ver. 5.65, however, this selection and ɾAbsolute or Relative
entry process for qualifier MRM transitions now provides
for automatic selection and entry as reference ions.
(4)
< Examples of New Features >
(1) Multiple reference ions are automatically entered
(desired transitions can be selected and changed
using drop-down menu).
(2) The ion ratio of the STD is automatically set as the
reference value. Fig. 3 Reference Ion Setting Window
(3) A different allowable width of relative ion ratio can
be set for each reference ion.
(4) The identification range (%) is automatically
calculated from the ion ratio, allowable width and
reference ion mode.

(×10,000)
73:255.00>209.20(+) Sample 1 Identification
73:255.00>105.10(+) Indicator Retention Time Reference Ion Ratio -1 Reference Ion Ratio -2
1.0 73:255.00>77.10(+) Not identified
Judgment   
0.0
(×10,000) 8.0 8.5 9.0
73:255.00>209.20(+) Sample 2 Identification
2.5 73:255.00>105.10(+) Indicator Retention Time Reference Ion Ratio -1 Reference Ion Ratio -2
73:255.00>77.10(+) Not identified
Judgment   
0.0
8.0 8.5 9.0
(×10,000)
73:255.00>209.20(+) Sample 3 Identification
5.0 73:255.00>105.10(+) Indicator Retention Time Reference Ion Ratio -1 Reference Ion Ratio -2
73:255.00>77.10(+) Identified
2.5 Judgment   
0.0
8.0 8.5 9.0

Fig. 4 Example of Peak Determination Using Multiple Reference Ions

Table 2 Analytical Conditions
Column : Shim-pack XR-ODS Ⅱ (2.0 × 75 mm, 2.2 µm)
Mobile Phase A : 0.1 % Formic acid – Water
Mobile Phase B : Acetonitrile
Time Program : 1 %B (0 min) → 15 %B (1 min) → 40 %B (6 min) → 100 %B (10 - 13 min) → 1 %B (13.01 - 16 min) (12.01 - 15 min)
Flowrate : 0.2 mL/min.
Injection Volume : 2 µL
Oven Temperature : 40 °C
Ionization Mode : ESI (Positive / Negative)
Probe Voltage : +4.5 kV / -3.5 kV
Neburizing Gas Flow : 3.0 L/min.
Drying Gas Flow : 10.0 L/min.
Heating Gas Flow : 10.0 L/min.
Interface Temperature : 400 °C
DL Temperature : 200 °C
Block Heater Temperature : 400 °C

First Edition: Jan. 2015

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