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Taste Components (Search by Metabolome Analysis)

Metabolites in biological samples are widely involved in the metabolome analysis approach combines a triple quadrupole
flavor and aroma of food products. Accordingly, metabolome mass spectrometer (GC-MS/MS, LC-MS/MS), which provides Evaluation of Various Beer Beverages by Metabolome Analysis
Evaluation of Various Beer Beverages by Metabolome Analysis
analysis methods, which provide a comprehensive analysis of excellent separation, quantitation, and stability, with a
Hydrophilic metabolites in five beer beverages were analyzed using GC-MS/MS and LC-MS/MS, and the database for primary metaboli
te analysis.
metabolites, are being actively applied to research and database for primary metabolite analysis. This approach is used Hydrophilic metabolites in five beer beverages were analyzed using GC-MS/MS and LC-MS/MS, and the database for primary metabolite analysis.
As a result of a multivariate analysis (principal component analysis) of the data, it was confirmed that the five samples showed specific clusters in
development of food products, for such applications as in the field of food development as an efficient and effective As a result of a multivariate analysis (principal component analysis) of the data, it was confirmed that the five samples showed specific clusters in
the score plot, and based on the loading plot, sugar, amino acid and other characteristic components with a large contribution to the cluster forms
the score plot, and based on the loading plot, sugar, amino acid and other characteristic components with a large contribution to the cluster forms
analyzing taste components and determining the stability of the method of searching for taste components. were identified.
quality of fermented foods. The wide target quantitative
(×10,000,000)
1.75 Food Texture
Gas Chromatograph Mass Spectrometer
High Peptide LC-MS/MS 1.50 Gas Chromatograph Mass Spectrometer
GCMS-TQ8050
GCMS-TQ8050
Coenzymes, nucleotides, lipids 1.25
Food Texture
Steroids, vitamins 1.00
Terpene Nucleosides, sugar phosphates GC-MS(/MS) 0.75
Molecular Weight
Hydrocarbon Sugars, amino acids, organic acids 0.50
Esters Fatty acids 0.25
Ketones 7.5 10.0 12.5 15.0 17.5 20.0
MRM Chromatogram
Alcohol
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
GC-MS(/MS) OA_TMS_BPX5_23min_V3_MRM_20151124.M1 (PCA-X)
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Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
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Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
Colored according to model terms
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Ale beer
GC-MS(/MS) LC-MS/MS 10,000 0.3
Mannose-meto-5TMS(1)
・Hundreds of components can be analyzed simultaneously ・Specific metabolites (up to 100 components) can be 5,000 Non-alcoholic 0.2
with a single measurement. measured easily. t[2] 0 beer Low-malt beer p[2] 0.1
・Excellent robustness ・High molecular weight, non-volatile metabolites can also Lager beer 0 5-Oxoproline-2TMS
be measured. -5,000
Lager beer -0.1
containing rice
-10,000
-0.2
Database for Primary Metabolite Analysis -15,000 -0.3 Components of Deliciousness
Isomaltose-meto-8TMS(1)
-0.4
-20,000 -0.3 -0.2 -0.1 0 0.1 0.2 0.3
GC/MS Database—Smart Metabolites Database -20,000 -15,000 -10,000 -5,000 0 5,000 10,000 15,000 p[1]
t[1]
Registered Compounds Derivatization Method Measurement Method Number of Compounds Registered R2X[1] = 0.432 R2X[2] = 0.31 R2×[1] = 0.432 R2×[2] =0.31
Score plot Loading plot
Organic acids, fatty acids, TMS Scan 568
amino acids, sugars, etc. MRM 475 Lager beer
Components of Deliciousness
Liquid Chromatograph Mass Spectrometer
Scan 50 LCMS-8060
Fatty acids Methylation
MRM 50
Amino acids EZ:faast Scan 33
LC/MS Database—LC/MS/MS Method Package Ver. 2
Registered Compounds Method Number of Compounds Registered
Metabolites, amino acids, nucleotides, etc. in the major metabolic pathways Ion pair reagent used 55 Low-malt beer
Amino acids, organic acids, bases, etc. PFPP columns used 97 Lager Low-malt Non-
Lager Ale (contains rice) beer alcoholic
Example of a Food Metabolomics Application
Item Target Component Sample Odors and Other Items
Taste Components Primary metabolites and dipeptides Liquors, beverages, fermented food Non-alcoholic beer
Deliciousness
Odor Volatile components products, agricultural crops
Agricultural crops, fermented foods,
Functional Components Secondary metabolites
Functionality herbal medicines
Odors and Other Items
Effects of Components Primary metabolites Blood, urine, cells
Fermented and Brewed Products Primary metabolites Fermented food products
Quality and Productivity
Plant Breeding (Agricultural Crops) Primary metabolites, phytohormones Agricultural crops MRM chromatograms Classification of beer type by hierarchical clustering analysis (HCA)
Degradation of Components (Mislabeled Volatile components, primary Source: Shimadzu Application News No. C134.
Distribution and Storage Food Products, Distribution, Storage Quality) metabolites Agricultural crops, food products
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