Page 11 - Food&Beverages-Food Development
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Fats and Oils  Bitter and Astringent Components (Functional Components)


 With meats and fish, in addition to being gustatory   and oils, differ depending on the type of fatty acids derived   The bitterness and astringency of coffee and tea, and the   and metabolic stimulation effects.
 components, fats and oils, which are components of adipose   from the raw ingredients, and desaturation and oxidation. In   bitterness of beer, chocolate, and edible wild plants are   Analytical instruments such as liquid chromatographs are used
 tissue, and do not have an intrinsic chemical flavor, elicit   developing more delicious food products, it is important to fully   indispensable elements of the deliciousness of food products.   in the field of food development as analysis methods for the
 deliciousness by imparting a mellow taste. In addition, fats and   grasp the content and chemical properties of these fats and oils.  Polyphenols, alkaloids and other compounds are known to be   various gustatory components and functional components in
 oils give a crispy texture to foods, and create a creamy texture   Gas chromatographs, infrared spectrophotometers and other   bitter and astringent components. These compounds are also   food products.
 by incorporating air in the form of tiny bubbles, so they play an   analytical instruments are used in the field of food development   widely known to be functional components, with antioxidant
 important role in the physical structure of food products.  as tools for the analysis of fats and oils.
 The characteristics of triacylglycerol, the main component in fats

            Ultra High Performance Liquid Chromatograph                                                                Food Texture
            Ultra High Performance Liquid Chromatograph
            Nexera X2
            Nexera X2
 Gas Chromatograph
 Nexis GC-2030
 Food Texture
 Analysis of Fatty Acids in Fish Oil
 In addition to bolstering deliciousness, components of fatty acids   High Performance Liquid Chromatograph (HPLC)
 contained in fish, such as EPA and DHA, are known for their
 functionality.                                                 This instrument measures the amount of various components in samples.
 This example shows the analysis of fatty acid methyl esters in fish   The components in the sample are separated, and then quantified.
 oil. It was confirmed that various fatty acids could be detected   It is very versatile, and is widely used for analysis in the food sector. In
 with high separation.                                          addition to instrument configurations suited to the sample and
                                                                application, our lineup features models aimed at high-speed analysis.



               Analysis of Chlorogenic Acid in Coffee
 1=
 C 18
 C 16
               Chlorogenic acid is one of the many bitter and astringent components in coffee.
               It is a polyphenol compound widely distributed amidst the higher plants. In   200  ■Peaks
 Gas Chromatograph  C 20 1=  C 20 5=  1=  addition, recently, its antioxidant capabilities have become a focus of interest,   1. Chlorogenic Acid
 1=  C 22
 C 16  1=  6=  and a variety of related research is being pursued.
 This analytical instrument measures the content of various components   2=  4=  C 24 +  C 22  2  2. Caffeine
 C 14  C 18  C 18 C 18  This example shows the analysis of chlorogenic acid in commercially available
 in samples. The components in the sample are separated, and then   C 18 3=  C 20 4=  C 22 5=
 qualified and quantified.  0.0  2.0  4.0  6.0  8.0  10.0  12.0  14.0  16.0  18.0  20.0  22.0  24.0  26.0  28.0  30.0  32.0  34.0  36.0  38.0  instant coffee. It was confirmed that trace amounts of chlorogenic acid can be   mAU  100
 It is capable of high-sensitivity trace quantity analysis, and is widely used   (min)  specifically detected.  1      Components of Deliciousness
 in the fields of food development and quality control.
               Source: Shimadzu Application News No. L306.             0
                                                                        0                                5                                10                              15
                                                                                         min
 Fourier Transform Infrared Spectrophotometer
 Components of Deliciousness
 IRTracer-100  Analysis of Fatty Acids in Red Wine  Analysis of Catechins in Green Tea
               Catechins are widely known as astringent components.  mAU
 Food products consist of mixtures of a variety of compounds.   At the same time, many research reports have highlighted their ability to lower   3  ■Peaks
 Accordingly, combining various analysis methods is an effective   body fat and cholesterol in the blood, and their antioxidant and   1. Gallic Acid     8. Gallocatechin Gallate
 approach to the analysis of components.  cancer-preventing effects. These functional properties are also a focus of   7  2. Gallocatechin    9. Epigallocatechin 3-(4” -O -methyl)gallate
                                                                                             10. Epigallocatechin 3-(3” -O -methyl)gallate
 In this example, red wine is filtered using a membrane filter. The   attention.    3. Epigallocatechin  11. Epicatechin Gallate
                                                                                    4. Caffeine
 components are then separated by a liquid chromatograph, and   This example shows the high-speed simultaneous analysis of 14 catechins in   4  5. Catechin  12. Cafechin Gallate
 the separated materials are measured by the infrared   commercially available green tea, including methyl catechin, which has been   100  6. Epicatechin  13. Epicatechin 3-(4” -O -methyl)gallate
                                                                                    7. Epigallocatechin Gallate
                                                                                             14. Epicatechin 3-(3” -O -methyl)gallate
 spectrophotometer. Using a library search tartaric acid (a fatty   reported in the research to have anti-allergy effects. It was confirmed that the   6
 acid) was identified.  various catechins can be analyzed quickly and with high accuracy.
                                                                           2
                                                                           (1)           10  11
                                                                              5                         14
                                                                       0
               Source: Shimadzu Application News No. L376.
 %                                                                     0.00   0.50  1.00   1.50   2.00   2.50 min
 100.00  100.00
 Fourier Transform Infrared Spectrophotometer  A  88.00  88.00
 This instrument mainly performs structural estimations of organic   C  76.00  76.00
 compounds. When molecules are irradiated with infrared rays, the                                                      Odors and Other Items
 absorbance of the infrared rays differs depending on the structure of   B  64.00  64.00
 the molecules. Quantitation and structural estimations of compounds   52.00  52.00
 are performed by investigating the absorbance (infrared absorption   40.00  40.00  Analysis of Components in Food Products
 spectrum) pattern.  5  -  10  -  15  -  20  -  4000.0        3000.0  2000.0          1500.0          1000.0          500.0 400.0    cm −1
 Measurements can be performed with relatively easy pretreatment and   The information provided in this brochure is just a fraction of what is available. In addition to the information provided in this catalog, Shimadzu has a wealth of experience
 a wide range of samples can be applied.  in analyzing components in food products, including gustatory components, and components with functional properties (such as isoflavone, resveratrol, gingerol, flavanone,
 Odors and Other Items
             and lycopene).
             For details, contact your Shimadzu representative.


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