Page 16 - Cannabis&Hemp Industrial-Hemp-Brochure

Seed: Foods

Liquid Chromatography / Mass Spectrometry

Analysis of Vitamins
Though many AOAC Official Methods for vitamins employ
microbiological assays or LC with fluorescence detection, use of
LC-MS/MS techniques in this field is rising because they offer
advantages over these other methods, including specificity,
speed, ability to detect impurities, and broad applicability. The
Shimadzu LCMS-8040 with the Nexera UHPLC provides excellent
instrumentation for fat-soluble vitamin analysis.

450000
Vitamin E Acetate Vitamin A Palmitate
400000
350000 beta-Carotene
300000
250000
200000
Vitamin K 1 Vitamin D 3
150000
100000
50000
0
0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 3.00 3.25 3.50 3.75 4.00 4.25 4.50 4.75 5.00 5.25 min

MALDI-TOF MS / Protein Sequencing
Protein Analysis
Traditional Edman sequencing avoids mass dependency and the use
of databases by analyzing each amino acid from the N-terminus one
at a time in sequence. Unfortunately, Edman has its own limitations
in providing high sequence coverage. Mass spectrometry has become
an indispensable tool for researchers looking to sequence peptides.
Although effective in many cases, sequencing by In Source Decay (ISD)
faces a few challenges, e.g., its ability to provide reliable sequence
information including isobaric amino acids, database dependency, and
low molecular weight interferences.

Combining the powerful capabilities of MALDI-TOF MS (MALDI-8020)
and Edman sequencing (PPSQ -50A Gradient System) provides a
TM
more accurate and complete N-terminal sequence of peptides.

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