Page 15 - Cannabis&Hemp Industrial-Hemp-Brochure

Seed: Foods

Gas Chromatography

Analysis of Fatty Acids
Analysis using Gas Chromatography (GC) is a suitable measurement technique, as GC has a high separation capability. In
this technique, the fatty acids are first derivatized by methyl esterification to produce fatty acid methyl esters (FAME) with
sufficient volatility. However, localization of the double bond position in unsaturated FAMEs is difficult, even when using
mass spectrometry.

To overcome this difficulty, a new analysis technique was developed: Solvent
Mediated Chemical Ionization (SMCI). It is a soft ionization method using an
organic solvent. The new Shimadzu SMCI unit makes it possible to introduce
acetonitrile, which is necessary in this double bond localization technique,
into the analytical instrument.

Analytical & Testing Solutions for Industrial Hemp 15