Page 9 - Shimadzu Journal vol.9 Issue2

“It is excellent and extremely beneficial in support of our research and development process.” | INSIGHT from CUSTOMER

Dr. Shivam Mukherjee

What are the major challenges in
performing analysis for cell culture
components of spent media?

The major challenges in identifying components
in spent media are extraction methods that are used
to monitor different class of analytes (amino acids,
nucleic acids, vitamins) and their inherent differences
in concentration.

How is Shimadzu LC-MS along with
cell culture profiling method package
helping you in your research?

Usage of Shimadzu cell culture package provides a
number of advantages with respect to simultaneous
analysis of a wide range of components including ones Thank you again for your time today.
which might be present in less concentration. A 17-min Finally, could you share any requests
run allows identification of 95 components using that you have with respect to analytical
the MRM principle which distinguishes components and measuring instrument vendors?
that are difficult to monitor using conventional LC tech-
niques. That’s why we chose Shimadzu. We have a beneficial relationship with Shimadzu instru-
ments and related method packages. We would like to
How would you describe the value continue this and receive your updates on a regular
that Shimadzu technology added to basis which will be helpful in our research.
your research project?

Shimadzu CCP package combined with the ultrafast Dr. Shivam Mukherjee did his un-
and sensitive LCMS-8060 is a very powerful and dergraduate studies in Chemistry and
useful tool that we employ to have a quick identifi- then proceeded to do his doctoral
studies at Stony Brook University,
cation of components present in media/feed and
New York. Post completion of his
spent media. The range of compounds present thesis, he returned back to India and
in the method package and the short turn- joined TCG Lifesciences in Kolkata.
around time in identification helps the He joined Intas Biopharma Ltd. in
2019 and since then has been work-
process development teams in screen-
ing in the R&D Analytical Development Laboratory where he &
ing of multiple media and feed com- his team's work focusses on developing LC-MS methods used in
binations, thus ensuring the best identification and characterization of biomolecules.
possible one is carried forward in
upstream process development.

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