Page 6 - Shimadzu Journal vol.9 Issue2

“It’s a strong, reliable, and synergistic bonding.” | INSIGHT from CUSTOMER

Ms. Shraddha Mane

make available world-class media & feed required
for the manufacture of Biosimilar at a very afford-
able price as compared to imported media and
feed. My team comprising of Ms.Gauri Page, Ms.
Soni Shukla, and Ms. Mausami Bhattacharya
worked very hard under the guidance of the direc-
tors of the cell biology division, Dr.Vishal G. Warke,
and Dr.Priti V. Warke.
The award was presented by Shri M. Venkaiah
Naidu, Hon’ble Vice President of India, and Dr.
Harsh Vardhan, Hon'ble Union Minister for Science
& Technology, Earth Sciences and Health & Family
Welfare, India. The distribution of the award was
done through a virtual ceremony during the Global
Bio- India 2021 on 3rd March 2021.

What according to you, are the advantages of Apart from the basic components of
chemically defined media over conventional chemically defined media like amino
serum-based media? acids, vitamins, etc. what are the other
components that can be present in it?
Chemically defined media has the following advantages
over serum-containing medium: Other than basic components present, media also con-
Cost-effectiveness tains trace elements, lipids, and fatty acids which are ben-
Lot to lot consistency eficial for cell proliferation. Also, during the cell culture
Free of any adventitious agent process, there may be secretion of toxic organic elements,
Good productivity and identifying such elements is necessary since they may
Easy downstream process hamper cell viability and/or productivity. Overall, the anal-
ysis of such essential and trace elements helps to design
Could you briefly explicate how Shimadzu’s the apt media and feed for commercial cell lines.
Cell Culture Profiling (CCP) method package
along with LC-MS is useful for developing Which analytical tool you are using to
chemically defined media? determine the concentration of such trace
elements and inorganic salts in media?
Cell culture media analysis is an integral part of biopro-
cess technology. Right from the initial raw material Shimadzu’s ICPMS-2030, because of its high sensitivity,
analysis to in-process media components monitoring, it is an appropriate instrument for the determination of
helps understand the cell growth cycle and productivity. trace elements and inorganic salts content in the media.
Hence comprehensive analysis methods like the CCP
method package using LC-MS/MS can help us get a What is the importance of fresh media
better perspective towards media components selec- and spent media analysis? How do you
tion, its impact on cell growth, and in terms of produc- find Shimadzu LCMS-8045 and ICPMS-2030
tivity. It helps for tracking the changes in chemical profile useful for this application?
during fermentation and deciding the feed composition
and time. The Ultra-Fast Mass Spectrometer (UFMS) Analysis of fresh media is necessary to assess the compo-
technology of Shimadzu mass spectrometers like fast nents present in the medium before starting the cell cul-
scanning speed, polarity switching time, and negligible ture. Whereas spent media analysis will help in the
cross talk helps in simultaneous estimation of all the understanding the changes in media components, analy-
media compounds in a single run. sis of nutrient’s consumption trend and its impact on cell

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