Page 22 - Shimadzu Journal vol.5 Issue1

Bioanalysis

Discussion and conclusion

We have developed a metabolic methodology by using LCMS-8050
(SHIMADZU) and Pathpod pathway mapping system, to analyze the
changes and temporal pattern correlativity of comparative intracellular
metabolite amounts. Our results indicated that most amino acids
showed negative correlations in the comparison of intracellular
metabolic temporal patterns between growth phase and non-growth
phase, except asparagine, aspartate, and glutamine. Based on the
carbohydrate analysis in CMPs including glycolysis and TCA cycle
reported by other studies, amino acid metabolism additionally provides
information for animal cell cultivation and biopharmaceutical industry.

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