Page 20 - Shimadzu Journal vol.5 Issue1

Bioanalysis

Table 2 MRM Transitions for MS

Amino acid Organic acid
Conpound name m/z Qualifier ion Scan time (min) Conpound name m/z Qualifier ion Scan time (min)
4-Hydroxyproline 132.10>86.05 132.10>68.05 3.239 2-Aminobutyric acid 104.10>58.05 104.10>41.05 4.763
Alanine 89.90>44.10 3.403 2-Ketoglutaric acid 144.90>101.10 144.90>57.05 5.750
Arginine 175.10>70.10 175.10>60.10 5.000 4-Aminobutyric acid 104.10>87.05 104.10>45.10 5.000
Asparagine 133.10>87.15 133.10>28.05 3.199 Aconitic acid 172.90>85.05 172.90>129.10 5.000
Aspartic acid 134.00>74.05 134.00>88.10 3.197 Adenylsuccinic acid 464.10>252.10 464.10>162.00 5.000
Citrulline 176.10>70.05 176.10>159.05 3.463 Argininosuccinic acid 291.00>70.10 291.00>116.05 5.861
Cystathionine 223.00>88.05 223.00>134.00 3.230 Cholic acid 407.20>343.15 407.20>345.25 5.000
Cysteine 122.00>76.05 122.00>59.00 3.349 Citric acid 191.20>111.10 191.20>87.05 5.000
Cystine 241.00>151.95 241.00>73.90 3.156 Fumaric acid 115.00>71.10 115.00>26.95 5.000
Cytosine 112.00>95.10 5.000 Isocitric acid 191.20>111.10 191.20>73.00 5.000
Dimethylglycine 104.10>58.05 104.10>44.05 3.405 Lactic acid 89.30>89.05 4.977
Glutamic acid 147.90>84.10 147.90>56.10 3.404 Malic acid 133.10>114.95 133.10>71.15 4.628
Glutamine 147.10>84.15 147.10>130.10 5.000 Nicotinic acid 124.05>80.05 124.05>78.05 5.000
Glycine 75.90>30.15 3.171 Ophthalmic acid 290.10>58.10 290.10>161.10 5.000
Histidine 155.90>110.10 155.90>56.10 4.949 Orotic acid 155.00>111.10 155.00>42.10 4.469
Homocysteine 136.00>90.10 136.00>56.10 5.000 Pantothenic acid 220.10>90.15 220.10>72.05 5.000
Homocystine 269.00>136.05 269.00>88.00 5.000 Pyruvic acid 86.90>87.05 86.90>42.95 4.803
Isoleucine 132.10>86.20 132.10>69.15 5.000 Succinic acid 117.30>73.00 117.30>99.05 5.000
Leucine 132.10>86.05 132.10>30.05 5.000 Taurocholic acid 514.20>107.10 514.20>124.05 5.000
Lysine 147.10>84.10 5.000 Uric acid 167.10>123.95 167.10>96.20 5.000
Methionine 149.90>56.10 149.90>104.10 5.000
Ornitine 133.10>70.10 133.10>116.05 4.840 Purines
Phenylalanine 166.10>120.10 166.10>103.10 5.000 Conpound name m/z Qualifier ion Scan time (min)
Proline 116.10>70.15 116.10>28.05 4.707 Allantoin 157.00>97.10 157.00>42.05 3.254
Serine 105.90>60.10 3.209 Hypoxanthine 137.00>55.05 137.00>110.00 5.000
Symmetric dimethylarginine 203.10>70.15 203.10>71.10 5.000
Threonine 120.10>74.15 120.10>56.05 3.237 Choline
Tryptophan 205.10>188.15 205.10>146.10 5.000 Conpound name m/z Qualifier ion Scan time (min)
Tyrosine 182.10>136.10 182.10>91.10 5.000 Acetylcholine 147.10>87.05 147.10>88.05 5.000
Valine 118.10>72.15 118.10>55.05 5.000 Choline 104.10>60.05 104.10>45.10 5.000
Amino acid derivative Peptide
Conpound name m/z Qualifier ion Scan time (min) Conpound name m/z Qualifier ion Scan time (min)
Carnitine 162.10>103.05 162.10>60.10 5.000 5-Glutamylcysteine 251.10>84.10 251.10>122.10 5.000
Dopa 198.10>152.10 5.000 Carnosine 227.10>110.05 227.10>156.05 5.000
Kynurenine 209.10>192.05 209.10>94.10 5.000 Glutathione 308.00>179.10 5.000
S-Adenosylhomocysteine 385.10>134.00 385.10>136.05 5.000 Oxidized glutathione 611.10>306.00 611.10>143.05 5.000
S-Adenosylmethionine 399.10>250.05 399.10>136.10 5.000
Serotonin 177.10>160.10 177.10>77.05 5.000 Lactam
Conpound name m/z Qualifier ion Scan time (min)
Nucleotide Creatinine 114.10>44.05 5.000
Conpound name m/z Qualifier ion Scan time (min)
Adenosine 3',5'-cyclic monophosphate 330.00>136.05 330.00>119.10 5.000 Catecholamine
Adenosine monophosphate 348.00>136.05 348.00>97.10 5.000 Conpound name m/z Qualifier ion Scan time (min)
Citicoline 489.10>184.10 489.10>264.05 3.421 Dopamine 154.10>91.05 154.10>137.05 5.000
Creatine 132.10>44.05 132.10>90.05 5.000 Epinephrine 184.10>166.10 184.10>77.00 5.000
Cytidine 3',5'-cyclic monophosphate 306.00>112.10 5.000 Norepinephrine 170.10>152.15 170.10>107.10 5.000
Cytidine monophosphate 324.00>112.05 324.00>95.00 4.531
Guanosine 3',5'-cyclic monophosphate 346.00>152.05 346.00>135.05 5.000 Vitamine
Guanosine monophosphate 364.00>152.05 364.00>135.00 4.912 Conpound name m/z Qualifier ion Scan time (min)
Thymidine monophosphate 322.90>81.10 322.90>207.10 5.000 Niacinamide 123.10>80.05 123.10>53.10 5.000
Nucleoside Base
Conpound name m/z Qualifier ion Scan time (min) Conpound name m/z Qualifier ion Scan time (min)
Adenosine 268.10>136.05 268.10>119.00 5.000 Adenine 136.00>119.05 136.00>65.00 5.000
Cytidine 244.10>112.05 244.10>95.00 5.000 Guanine 150.00>133.00 150.00>66.10 5.000
Guanosine 284.00>152.00 284.00>135.00 5.000 Thymine 127.10>54.05 127.10>110.05 5.000
Inosine 269.10>137.05 269.10>118.95 5.000 Xanthine 151.00>108.00 151.00>42.00 5.000
Thymidine 243.10>127.10 5.000
Uracil 113.00>70.00 5.000 Aminothiol
Uridine 245.00>113.05 5.000 Conpound name m/z Qualifier ion Scan time (min)
Cysteamine 78.10>61.05 5.000
Amine derivative
Conpound name m/z Qualifier ion Scan time (min)
Histamine 112.10>95.05 112.10>41.05 5.000
Coenzyme
Conpound name m/z Qualifier ion Scan time (min)
FAD 786.15>136.10 786.15>348.10 5.000
FMN 455.00>97.00 455.00>78.90 5.000
NAD 663.10>541.05 663.10>540.10 5.000
Lipid
Conpound name m/z Qualifier ion Scan time (min)
Acetylcarnitine 204.10>85.05 204.10>60.10 5.000

20