Page 5 - Shimadzu Prominence Nano

Expandability

Prominence nano provides ultra flexibility

Easy to combine with MALDI-TOF MS systems – AccuSpot MALDI spotting device –
The Prominence nano system can be used with an online connected nano-ESI mass spectrometer or with a MALDI TOF mass
spectrometer with a MALDI plate spotting device.
For the accurate spotting of each chromatographic peak onto a MALDI plate, flow rates at about several hundreds of nL/minute are
commonly applied; thus, LC with low flow rate precision is required. The Prominence nano is an ideal system for this purpose.

Retention time: 13.93 min
5.0 10.0 15.0 20.0 25.0 30.0 35.0 min Spot: #70
Separation of digested BSA sample (500 fmol as protein) (UV chromatogram)

Analytical conditions
Detection UV 220 nm
Column MonoCap for Fast-flow (250 mm L. x 100 μm I.D.)
Mobile phase A) Water / Acetonitrile / TFA = 95/ 5/0.1 (v/v)
B) Water / Acetonitrile / TFA = 10/90/0.1 (v/v) Retention time: 11.16 min
Spot: #56
Gradient elution
Flow rate 1 μL/min
Temperature Ambient
Trapping column ODS (1 mm L. x 0.5 mm I.D.)
Spotting period Every 12 seconds
Spotting volume for one spot: 200 nL except matrix solution

Proteome analysis system combined with a Shimadzu mass spectrometer
– Providing maximum performance and a total solution –

When connected to a Shimadzu mass spectrometer, the Prominence nano system demonstrates its maximum performance.
A total system configuration, comprising the Prominence nano and either the LCMS-IT-TOF MS with a nano-ESI interface or a MALDI-
TOF MS with the AccuSpot MALDI spotter, provides authentic results and a total solution for proteomic analyses.
* Prominence nano can operate stand-alone or with a mass spectrometer, including those manufactured by other vendors.

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