Page 2 - Shimadzu Prominence Nano

Features

Uncompromising core performance

for high-precision proteome analysis

The Reflux Flow Control (RFC)* System offers superior flow-rate precision in the nano
flow-rate range, making nano flow-rate gradient analysis with higher reproducibility
possible. *Patent pending

Unnecessary solvent consumption is eliminated because a split drain after 2-liquid
mixing is not required.
In the nano flow-rate range, stable solvent delivery and solvent consumption volume are important. The LC-20ADnano equips nano
flow-rate control mechanisms for each pump independently. The flow rate is controlled by feedback from signals from the high-precision
nano flow sensor, so that precise flow rates are always assured. Additionally, the RFC system offers stable solvent delivery and low
solvent consumption, so that unnecessary solvent consumption is eliminated and the impact on the environment is minimized. The nano
flow sensor features a precise temperature control to minimize the influence of room temperature variation on the flow rate.

Pump A: LC-20ADnano
Reflux flow pass

Reflux block
Primary flow Nano flow sensor
delivery unit
Mobile phase A Feedback control

Pump B: LC-20ADnano Reflux control unit
Reflux flow pass

Reflux Flow Control (RFC) System
Primary flow Reflux block Nano flow sensor
delivery unit Most of the mobile phase coming from the pump is rerouted to
the inlet of the pump through the reflux path. The speed of the
Mobile phase B Feedback control
pump is controlled so that the flow-rate detection value from the
flow sensor is the same as the set value.

FCV nano: a Shimadzu-developed nano valve – Superior performance and durability –
Shimadzu has developed a nano valve, which is required for trap injection and for 2-
dimensional LC in nano LC. Volume between ports is as low as 25nL, and peak
broadening is virtually zero in the nano flow-rate range. By combining a surface
hardened stator and a PEEK rotor, low adsorption and high durability are achieved.
Particles derived from the switching valve movement are minimized and clogging in
nano flow paths is suppressed.
Adsorption of the analytical sample is also suppressed. The FCV nano is an optimized
switching valve for bio sample analysis in the nL/min level.
PEEK is a registered trademark of Victolex plc (UK).

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