Page 3 - Shimadzu Prominence Nano

Graphically assisted software operation

Instrument control through an easy-to-understand GUI

Nano-Assist control software
– 2D LC operation made easy –

Complicated gradient programming for two-dimensional HPLC is made easy by
the Nano-Assist’s graphical user interface. Just input the parameters to have the
method file created and downloaded to the instruments. Visual presentations of
flow-line connections and gradient curves help prevent operation errors.

2D LC setting window of Nano-Assist

High peak resolution with 2D analysis Samples such as
enzymatically digested
proteins are introduced
The Prominence nano’s online 2D separation system combines ion exchange into the first dimension.
and reversed-phase modes as illustrated at right. Each separation mode works The sample is separated
in the first dimension.
independently, and the combination of the two modes gives the best separation Each of the fractions
from 1D is trapped
efficiency. The Prominence nano 2D system can constitute an online proteome on the trap column.
analyzer when coupled to an LCMS with a nano ESI interface or to a MALDI
spotter.

Second-
dimensional
separation

Schematic diagram of 2D LC separation
Easy-to-use 1D system

The one-dimensional nano LC system is a useful tool for identifying proteins pre-
separated with SDS-PAGE. The 1D nano LC system offers simple operation,
and the precise solvent delivery of the LC-20ADnano and the low dead volume
of the FCV nano maximize the system’s performance. Like the 2D system, the
1D system is operated using the dedicated software, Nano-Assist, which
provides easy operation and prevents possible mistakes in operation settings.

1D LC setting window of Nano-Assist
Autosampler with a total-volume injection method
– No waste of samples –
The combination of the proven performance of the SIL-20AC and the low dispersion
of the FCV nano valves allows analysis with a minimal injection volume. The total-
volume injection-type autosampler requires no extra sample volume, and the trapping
column flow line with the FCV nano minimizes peak broadening, providing maximum
sensitivity with the smallest sample volume. The SIL-20A also has an excellent
reputation as a low-carryover autosampler, reducing adsorption of sample
constituents to an absolute minimum, ensuring stable analysis with low sample SIL-20AC autosampler demonstrates
carryover. minimized sample carryover

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