Page 5 - Shimadzu Nexera lite inert

Comprehensive Solution

for Increasing Bioseparation Efficiency

Adsorption of biomolecules can occur not only within instruments, but also in vials, columns, and other Selecting Analytical Columns Based on Target Components and Objectives
equipment used during sample preparation. Shimadzu offers support for resolving such issues with
Shim-pack Series Columns
™
products that feature unique technologies for inhibiting adsorption.
Shimadzu offers columns optimized for variety of separation modes to analyze biomolecules, such as proteins, peptides and
nucleic acids.

Shim-pack Scepter ™
Nucleic Acids Reversed phase
Oligonucleotides
Shim-pack Bio IEX
Ion exchange

Shim-pack Bio Diol
Size exclusion
Shim-pack Arata ™
Shim-pack Velox ™
Shim-pack GISS
Proteins Peptide digests
Antibodies,
Antibody Drug Conjugates Aggregates and Fragments Shim-pack Bio Diol
Shim-pack Bio IEX
Charge variants
Shim-pack Bio HIC
$POKVHBUFT
State-of-the-Art Technology for Inhibiting Adsorption
and Increasing Recovery Rates

™
TORAST -H Bio Vial
Compounds are most likely to adsorb to vials they contact the longest. TORAST-H Bio vials offer exceptional resistance to
adsorption by biomolecules, which helps maximize recovery rates from valuable samples and increase sensitivity for analyzing
trace components.

uV uV
30000
Basic compound A : TORAST-H Bio Vial 4000 Basic compound B : TORAST-H Bio Vial
logP : 4.9 (100%) logP : 0.16 (100%)
20000 pKa : 9.4 : Other vendor’ s vial A 3000 pKa : 9.6 : Other vendor’ s vial A
(80%) (86%)
: Other vendor’ s vial B : Other vendor’ s vial B
(38%) 2000 (69%)
10000
1000
0
1.5 2.0 2.5 3.0 min 1.0 1.2 1.4 1.6 1.8 2.0 2.2 min
uV uV
30000 50000
Basic compound C : TORAST-H Bio Vial Basic compound D : TORAST-H Bio Vial
logP : 4.8 (100%) 40000 logP : 3.4 (100%)
20000 pKa : 9.4 : Other vendor’ s vial A pKa : 9.1 : Other vendor’ s vial A
(85%) 30000 (77%)
: Other vendor’ s vial B : Other vendor’ s vial B
10000 (36%) (18%)
20000
0 10000
0
1.0 1.5 2.0 2.5 min 1.1 1.2 1.3 1.4 1.5 1.6 1.7 min
Nexera lite inert
High Performance Liquid Chromatograph
4 5