Page 5 - Shimadzu MALDI-8030

SOLUTIONS FOR ALL YOUR APPLICATIONS SOLUTIONS FOR ALL YOUR APPLICATIONS

Food & Cosmetics Teaching & Research Labs

Perfect for...SCREENING of products EASY to USE means...smart choice for teaching laboratories
Whether it’s the presence of adulterants such as sunflower oil in extra virgin olive oil; regulated additives in As pioneers of MALDI-TOF MS, Shimadzu have designed the MALDI-8030 to be easy to use whilst still retaining
confectionery; or presence of halal components in cosmetics, the Shimadzu MALDI-8030 offers the ideal outstanding speed, robustness and mass accuracy. All these features facilitate the introduction of this technology
analytical solution. to students in bioanalytical teaching laboratories where the short time-to-result cycle of the MALDI-8030 will fit
in seamlessly with teaching sessions.

Food Colorants in Sweets/Candies
Genotyping for Teaching Demonstrations
Na +
451.008 O - Analysis of oligonucleotides on the MALDI-8030 is easy making it a good example for teaching laboratory
100 O
0.019 Da S demonstrations and avoiding the chores of time-consuming gels/ethidium bromide detection of oligonucleotides.
90 O
(error)
80 O
70 451.008 The dual-polarity ion source of Cystic fibrosis
100 80 N N O Na + the MALDI-8030 allows the 100 90 3657.89
60
% Intensity 50 % Intensity 60 40 O - O S O - flexibility to analyze 100 2759.02 ATTGGTGTT %Intensity 80 70 60 50 Na adduct
K adduct
90
(Phe508del CFTR gene)
40
20 oligonucleotides in negative 80 40 30 20 3696.51
30 70 10
0 mode (main spectrum), resulting
450 451 452 453 Allura Red AC (FD&C Red 40; E129) 0 3600 3650 3700 3750 3800
20 m/z 60 m/z
-
[M-H] m/z 451.027* in a cleaner mass spectrum
10 % Intensity 50 3657.02
*Calculated (loss of Na + ions) compared to positive ion mode
0 40 ATCTTTGGTGTT
250 300 350 400 450 500 550 600 650 30 (Normal CFTR gene)
m/z containing adducts (see inset).
20
The confectionery industry regulates colorants in sweets/candies prior to approval for release of the product. 10
The MALDI-8030 can detect FD&C and E number small molecule compounds (in this example, Allura red) using 0 2000 2200 2400 2600 2800 3000 3200 3400 3600 3800 4000 4200 4400 4600
m/z
a combination of a simple workflow and the class-leading MALDI linear TOF mass accuracy and resolution,
indicating it is a suitable analytical solution for screening this type of sample.
ADVANCED FEATURES means...suitable for research laboratories
Analysis of Pigments Used in Cosmetics/Lipsticks
Availability of advanced configurable features in MALDI Solutions software coupled with add-on tools for
dedicated applications.
Theoretical isotopic distribution
CI 15850 / 646.583
RED 7 407.007 100 646.698 100
100 90 90
CI 15985 / 80 80
90 YELLOW 6 70 644.701 648.695 70 644.595 648.573 Data Mining with Confidence Algorithm: Random Forest
Cross validation type: KFold
385.078 Adulterated EVOO 2x10 0 Training data: EVOO(47)
80 Lake 60 %Intensity 60 EVOO 1.5x10 0 Training data: Adulterated EVOO(30)
Training data: Sunflower oil (36)
50 50 Successful data analysis algorithms are key 2x10 0 Validation data: EVOO(26) 5
70 40 40 1x10 0 5 Validation data: Adulterated EVOO(52)
EVOO + 5% Sunflower oil
30 for research labs to filter out complex data 10 EVOO + 10% Sunflower oil 5
30 20 EVOO + 20% Sunflower oil 10 5 5
647.578
60 20 642.702 645.706 647.701 650.696 20 10 642.552 645.589 650.567 into meaningful outcomes or to identify 1x10 0 Sunflower 5x10 -1 20 20 10 10 5 5 5
% Intensity 50 10 0 643.708 651.696 0 642 643.544 646 m/z 648 650 651.565 non-vegan/ crucial but surreptitious biomarkers. This is 0x10 0 -5x10 -1 20 20 20 20 20 20 10 10 10 10 10 10 10 10 5 10 10 5 5 5 5 5 5
0x10 0
20
20
644
652
20
20
40
30 non-organic only possible with reproducible quality raw -1x10 0 20 20 20 20 20 10 10 10 10 5 5 5 5
CI 45380/ lipstick data. MALDI-8030 provides class-leading -1x10 0 -1.5x10 0
20
RED 22 mass accuracy and resolution, as well as -2x10 0
10 428.971 Lake -4x10 0 -3x10 0 -2x10 0 -1x10 0 0x10 0 1x10 0 2x10 0 3x10 0 4x10 0
486.940 642.552 -2x10 0
0 automated lockmass for data alignment in -4x10 0 -3x10 0 -2x10 0 -1x10 0 0x10 0 1x10 0 2x10 0 3x10 0 4x10 0 Machine learning (RandomForest)
350 400 450 500 550 600 650 700 750 800 analysis* was able to classify
m/z the MALDI Solutions software, to facilitate PCA score plot analysis results
The MALDI-8030 is easily able to determine the presence of pigments suitable for vegan/organic/halal this process. using eMSTAT Solution™ blinded 5% adulterated extra
virgin olive oil (EVOO) with
cosmetics (lipstick in example shown). The high quality data produces good similarity between the observed 100% accuracy.
and theoretical isotope distribution. This is useful as further confidence for identification of the pigment. * Requires eMSTAT Solution software
MALDI-8030
Dual-Polarity Benchtop Linear MALDI-TOF Mass Spectrometer