Page 6 - Shimadzu i-Series Method Transfer System

Achieves Seamless Method Transfer Process

Transfer Methods with a Single Button Operation—ACTO Function

The ACTO function also includes functionality for transferring HPLC methods to UHPLC methods. Just by loading
method files prepared on an existing system and entering information about the columns used, the optimized
methods will be presented for fast analysis. By selecting one of the flow lines, the function can also transfer methods
from UHPLC to HPLC.

Save as method files

The i-Series MT system has two flow lines matched to the UHPLC and HPLC delay volumes. In addition to simplifying
method transfer for customers using HPLC, this single integrated system also streamlines reviewing procedures for
increasing the speed of customers’ methods using UHPLC.

Flow line 1
HPLC Conditions mAU
125
Column : Shim-pack VP-ODS 1. 2-Hydroxy-4-Methoxybenzophenone-5-Sulfonic Acid
2. 2,2’,4,4’-Tetrahydroxybenzophenone
150 mm L. × 4.6 mm I.D., 5 µm 100 3. Ethyl p-Aminobenzoate
Mobile phase A : 20 mmol/L Phosphate buffer solution, pH2.5 4. 2,4-Dihydroxybenzophenone
Mobile phase B : Acetonitrile 75 2 5. 2,2’-Dihydroxy-4,4’-Dimethoxybenzophenone
Flow rate : 1.0 mL/min 50 3 4
Column temperature : 40°C 1 5
Time program 25
Time Unit Func Value
2.00 Pump B.Conc 30 0
25.00 Pump B.Conc 70
25.10 Pump B.Conc 30 0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
32.00 Controller Stop
5 times
Flow line 2 faster
UHPLC Conditions mAU
125
Column : Shim-pack XR-ODS II
75 mm L. × 3.0 mm I.D., 2.2 µm 100
Mobile phase A : 20 mmol/L Phosphate buffer solution, pH2.5
Mobile phase B : Acetonitrile 75
Flow rate : 0.97 mL/min
Column temperature : 40°C 50
Time program 25
Time Unit Func Value
0.44 Pump B.Conc 30 0
5.48 Pump B.Conc 70
5.50 Pump B.Conc 30 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 min
7.02 Controller Stop

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