Page 5 - Shimadzu DPiMS QT

Speedy Qualitative Analysis

Comparison of Sample Comparison of Qualitative
The qualitative analysis sequences for DPiMS QT Pretreatment Times Analysis Times
and Q-TOF LC/MS were compared. Using DPiMS 15 minutes
10 minutes
QT, it was possible to significantly reduce the
measurement time in comparison to conventional 5 minutes
methods using Q-TOF LC/MS.
0.3 minutes

Q-TOF LC/MS DPiMS QT Q-TOF LC/MS DPiMS QT

Comparison of Pretreatment Times

Q-TOF LC/MS Measurement: 10 minutes DPiMS QT Measurement: 5 minutes

Add 300 µL of acetonitrile, Centrifugation Instill the supernatant Mix 20 µL of whole blood Centrifugation Instill the supernatant
200 µL of water, and 100 µL of into an LC vial. and 200 µL of ethanol onto the sample plate.
whole blood to the Micro with 180 µL of water.
Volume QuEChERS kit.

Speedy Qualitative Analysis

Only a few dozen seconds are spent on DPiMS QT qualitative analysis. When performing qualitative screening
analyses, the time before results are checked can be reduced, improving procedural efficiency.

1000000 TIC of Whole Blood Sample with Additive 7.5e2 Results for Composition Estimation of Clotiazepam
646.32236
5.0e2
750000
2.5e2 647.32582 648.32755 Actual spectrum
0.0e0
500000
2.5e5 648.32829
5.0e5 647.32553 Theoretical spectrum
250000
7.5e5
1.0e6
646.32219
0.00 0.05 0.10 0.15 0.20 0.25 min 645.5 646.0 646.5 647.0 647.5 648.0 648.5 649.0 649.5 m/z
Single DPiMS QT Measurement: 0.3 minutes Implementing Accurate Mass and Composition
Estimation from the MS Spectrum
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