Page 5 - Cannabis&Hemp Hemp Testing Solution

Potency Testing by (U)HPLC

HPLC Analysis

The Shimadzu Hemp Analyzer has been extended to include the analysis of 15 cannabinoids in 15 minutes. The
cannabinoid list is the same as on page 4 with the addition of CBDVA, THCVA, CBCA, and CBL. The method uses
Shimadzu’s 11-part cannabinoid mix (220-91239-21) plus the four individual standards. For the full application,
request Shimadzu’s Application News No. HPLC-036.

Analysis of 15 Cannabinoids with the Shimadzu Hemp Analyzer

UHPLC Analysis

Shimadzu’s Nexera-i (LC-2040C 3D) UHPLC with a photodiode array (PDA) detector offers a solution to 16
cannabinoid analysis. The cannabinoid list is the same as on page 4 with the addition of CBNA, CBDVA, THCVA,
CBCA, and CBL. The method uses Shimadzu’s 11-part cannabinoid mix (220-91239-21) plus the five individual
standards. UV-Vis detectors provide the absorption at fixed wavelengths (i.e. 220 nm). The key advantage of the
PDA detector over a standard UV-Vis detector is that the full absorption profile from 190-400 nm is recorded. PDA
detectors are often used in the pharmaceutical industry to determine peak purity. For the full application, request
Shimadzu’s Application News No. HPLC-020.

mAU
275 Ch1 228nm,4nm Ch1 228nm,4nm CBDVA
250
CBDA
225
200 CBGA
CBN
175
CBDV
150 THCVA
125 CBC
CBG CBD
100 THCV CBNA THCA
d9-THC d8-THC CBL
75
50 CBCA
25
0
-25
0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 3.00 3.25 3.50 3.75 4.00 4.25 4.50
Analysis of 16 Cannabinoids with the Shimadzu Nexera-i 4.5 minute separation of 16 Cannabinoids by
(LC-2040C 3D) UHPLC with PDA UHPLC-PDA

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