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Potency Testing by (U)HPLC






            HPLC Analysis


            The Shimadzu Hemp Analyzer has been extended to include the analysis of 15 cannabinoids in 15 minutes. The
            cannabinoid list is the same as on page 4 with the addition of CBDVA, THCVA, CBCA, and CBL. The method uses
            Shimadzu’s 11-part cannabinoid mix (220-91239-21) plus the four individual standards. For the full application,
            request Shimadzu’s Application News No. HPLC-036.



























            Analysis of 15 Cannabinoids with the Shimadzu Hemp Analyzer

            UHPLC Analysis

            Shimadzu’s Nexera-i (LC-2040C 3D) UHPLC with a photodiode array (PDA) detector offers a solution to 16
            cannabinoid analysis. The cannabinoid list is the same as on page 4 with the addition of CBNA, CBDVA, THCVA,
            CBCA, and CBL. The method uses Shimadzu’s 11-part cannabinoid mix (220-91239-21) plus the five individual
            standards. UV-Vis detectors provide the absorption at fixed wavelengths (i.e. 220 nm). The key advantage of the
            PDA detector over a standard UV-Vis detector is that the full absorption profile from 190-400 nm is recorded. PDA
            detectors are often used in the pharmaceutical industry to determine peak purity. For the full application, request
            Shimadzu’s Application News No. HPLC-020.

                                                                      mAU
                                                                     275 Ch1 228nm,4nm Ch1 228nm,4nm   CBDVA
                                                                     250
                                                                                  CBDA
                                                                     225
                                                                     200           CBGA
                                                                                           CBN
                                                                     175
                                                                                CBDV
                                                                     150                  THCVA
                                                                     125                                CBC
                                                                                    CBG  CBD
                                                                     100              THCV     CBNA      THCA
                                                                                                 d9-THC  d8-THC  CBL
                                                                     75
                                                                     50                                       CBCA
                                                                     25
                                                                     0
                                                                     -25
                                                                     0.00  0.25  0.50  0.75  1.00  1.25  1.50  1.75  2.00  2.25  2.50  2.75  3.00  3.25  3.50  3.75  4.00  4.25  4.50
            Analysis of 16 Cannabinoids with the Shimadzu Nexera-i   4.5 minute separation of 16 Cannabinoids by
            (LC-2040C 3D) UHPLC with PDA                             UHPLC-PDA

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