Page 16 - Food&Beverages Beer

Ingredient Analysis – Ingredient Analysis –

Inorganic Water Contamination Mycotoxins in Beer Grains

Water, the universal solvent, is required to be free of 200.7, and 200.8, respectively. Mycotoxins often exist as contaminants in grains. To ensure the quantification of 14 mycotoxins in beer was developed*.
contaminates and odors that will affect the beer’s flavor, color, Shown in the graph is a 0, 0.2, 1 and 2 µg/L calibration curve for consumer food safety, manufacturers of food and beverages The 14 mycotoxins: patulin (PAT), nivalenol (NIV),
and taste. Heavy metal or inorganic contaminates, such as cadmium as well as the spectral line profile on an ICPE-9800. have to strictly manage risks from such contaminants. To deoxynivalenol (DON), aflatoxin (AF) B1, B2, G1, G2, T-2 toxin
Arsenic (As), Cadmium (Cd), Mercury (Hg) and Lead (Pb), can be The above techniques can also measure approximately 70 maintain high-quality food standards, it is essential to rapidly (T-2), HT-2 toxin (HT-2), zearalenone (ZON), fumonisin (FM) B1,
harmful to one’s health. These and other metals can be elements, such as aluminum, calcium, chromium, copper, iron, determine the concentrations of hazardous mycotoxins in foods B2, B3 and ochratoxin A (OTA) were determined in beer by
analyzed by atomic absorption (AA-7000), inductively coupled magnesium, nickel, phosphorus, potassium, and sodium. Salts or beverages. LC-MS/MS using a UHPLC system coupled to a LCMS-8030 triple
plasma emission (ICPE-9800), or inductively coupled plasma mass such as sodium can have an effect on the pH, precipitation, UHPLC-MS/MS offers the best combination of selectivity, quadrupole mass spectrometer with fast polarity switching and
spectrometry (ICPMS-2030) according to EPA methods 200.9, wort, and yeast. sensitivity, and speed for detection of these compounds in high-speed scanning.
complex matrices. In this study, a high-throughput method for

Cd 226.502
Cond 1
Ultra Fast
Cd 226.502 nm(1) 50000 Polarity Switching
35000 HT-2(+)
40000 FMB2(+)
30000 FMB1(+)
40000
25000 FMB3(+)
30000 30000 AFG2(+) AFG1(+)
20000
Intensity 15000 Intensity 20000 PAT(-) AFB2(+) T-2(+) OTA(+)
10000 20000 NIV(-) AFB1(+)
5000 10000 DON(-) ZON (-)
10000
0 r=0.99999
0
0.0 0.5 1.0 1.5 2.0 226.45 226.50 226.55 0
Concentration(mg/L)
2.0 3.0 4.0 5.0 6.0 7.0 min
Calibration curve of cadmium in water at 0, 0.2, 1, and 2 µg/L Spectral line profile of cadmium in water at 0, 0.2, 1, and 2 µg/L
by ICPE-9800 on ICPE-9800 Analysis of 14 mycotoxins in beer at 10 ppb by UHPLC-MS/MS*

O O O O O
O O O H O O O OH O
O O O O O
O
N O
H
O O O
O C H 3
O OC H 3 O OC H 3 O OC H 3 O OC H 3 C l
AFB1 AFB2 AFG1 AFG2 OTA
OH OH OH OH
O O O O OH O C H 3
O O O O O O O O O O O
OH OH OH C H 3 O C H 3 OH H O
OH OH O O O O O
NIV DON T-2 HT-2 ZON O
O O O
OH OH OH OH
O O O
OH OH OH
N H 2 OH O O N H 2 O O N H 2 OH O O O O
O O O O O O
O
OH OH O OH OH OH O OH OH O OH
H O O H O O H O O
FMB1 FMB2 FMB3 PAT
Structure of 14 Mycotoxins Shimadzu Triple Quadrupole LCMS-8050
Shimadzu’s ICPE-9800 Inductively Couple
Plasma for the analysis of metals

*ASMS 2012 Poster WP27-580: “High Throughput Quantitative Analysis of Multi-mycotoxin in Beer-based Drinks using UHPLC-MS/MS”; work performed using the previous
model, LCMS-8030

Shimadzu’s Total Support for Beer Analysis
16 Analytical and Testing Instruments for Beer 17