Page 14 - Food&Beverages Beer

Ingredient Analysis Ingredient Analysis –

Hops and Organic Water Contamination Organic Water Contamination

Pesticides can be found as contaminants in brewing water and glyphosate-drenched soil. This example shows results for the Many common organic water contaminates affecting beer method USEPA 524 for volatiles* and the liquid injection
grains. In particular, the herbicide glyphosate, which has quantitative determination of glyphosate, glufosinate and flavor are organic volatile and semi-volatile compounds such as method USEPA 525 for semi-volatiles. The chromatogram below
recently become the focus of the Munich Environmental AMPA (aminomethylphosphonic acid) using the LCMS-8050 naphthalene, hexachlorobenzene, and shows the analysis of 76 volatile compounds from 0.5 ppb to 20
Institute, has to be monitored carefully since it is probably triple quadrupole LC/MS/MS system according to DIN 38407-22 hexachlorocyclopentadiene. To analyze these and other specific ppb. Some countries permit headspace to replace purge and
carcinogenic. Elevated concentrations can be expected when of the German standard methods for the examination of water, organic compounds in water, follow the purge and trap GCMS trap methods.
glyphosate is applied to a crop shortly before the harvest, a waste water and sludge. This instrument was used instead of
process known as siccation. Siccation is already forbidden in HPLC with fluorescence detection. The LCMS-8050 allows the
Germany and Germany’s farm federation has reconfirmed that determination of glyphosate in concentrations as low as 0.1 650000 Helium Purge Gas
malt derived from glyphosate-sprayed barley has been banned; µg/L. Sample preparation was done after derivatization with 600000 Nitrogen Purge Gas
550000
however, glyphosate could have been used on farms prior to the 9-fluorenylmethyl chloroformate (FMOC-CL). 500000
450000
ban, meaning barley could still be grown in 400000
350000
300000
250000
Glyphosate Perchlorate Trimesium Diqaut Kasugamycine 200000
150000
O NH 2 O - CH 3 OH 100000
H 3 C P OH O Cl O H C S + CH + + H O OH 50000 0
OH O 3 3 N N 2.5 5.0 7.5 10.0 12.5 15.0
O
H O OH Overlay of 20 ppb volatile standards purged in helium and nitrogen Shimadzu GCMS-QP2010 SE Gas Chromatography Mass Spectrometer for
Chlormeqaut Paraquat ETU following USEPA method 524 the Analysis of Volatile and Semi-volatile Compounds
H 3 C O O
CH 3 Cl - NH
+ NH
N CH 3 H 3 C N + N + CH 3 H N H O NH NH
Cl CH 3 Cl - S O 2 For non-specific organic contaminates, the TOC-L Total Organic 0, 0.3, 0.5, 1, 2, 5, and 10 ppm with a coefficient of
Glufosinate Mepiquat Maleic hydrazide Nicotine Amitrole Carbon analyzer can provide analysis for total organic carbon, determination (r2) of 0.9996. The TOC-L has an optional
total inorganic carbon, and total carbon by EPA Method 415.3 . accessory for measuring Total Nitrogen (TN).
#
O NH 2 O H 3 C N
H 3 C P OH N N The 7-point calibration curve shown below includes standards at
+ H N
OH N H N
O
H 3 C CH 3 N NH 2
H
O N Cal. Curve
45.243
PTU Fosethyl aluminium
40
NH S O O CH
H 2 C H P 3 Al 3+ 30
O
NH 2 -
3 Area 20
10
0
0 2 4 6 8 10 11
Cono[mg/L]
TOC calibration curve from 0-10 ppm in drinking water Shimadzu TOC-L Total Organic Carbon
Analyzer for the Analysis of Non-specific Carbon

Shimadzu LCMS-8050 Triple
Quadrupole Mass Spectrometer

Application News “Highly Polar Pesticide Multi-Residue Analysis in Food Safety by LCMS/MS” * Shimadzu Scientific Instruments Application News No. GCMS-1502: “Guide to US EPA Methods 524.3 and 524.4 for Analysis of Volatile Organic Compounds in Drinking Water”
# Shimadzu Scientific Instruments Application News No. TOC-004: “EPA Method 415.3 - Determination of TOC in Source Water and Drinking Water”

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