Page 18 - Shimadzu-FoodSafety

Halal Testing Laboratory Data Management

The unauthorized blending of undeclared materials into food products has become a major concern among consumers. The increased demand for food safety analysis and measurement has led to a dramatic increase in the number of samples
Such action violates customer rights based upon economical and safety values and it is also a critical problem for communities being analyzed which, in turn, has resulted in demands for faster, easier-to-use instruments and software. With this
with ethical and religious beliefs. Shown below are the results from an analysis using a high-sensitivity LC-MS/MS method for background, faster, more efficient management of instruments and data is essential. LabSolutions is a network-compatible
ensuring halal food integrity by targeting more heat-stable porcine-specific peptide markers in processed food. analysis data system capable of meeting these needs.

LabSolutions CS – Total Laboratory Network

Peptide Spiking Percentage of Cooked Pork (%, wt) [n=4]
Marker Freely Accessible to the Analysis Network Recommended for the following customers
0.1 0.5 1.0 5.0
YDII + + + + Since all analytical data is managed in the database of a ・Facilities with a large number of instruments and users
LVVI + + + + server computer, LabSolutions CS can read data from any ・Facilities interested in enhancing procedural efficiency
personal computer on a network. In addition, analysis ・Facilities interested in enhancing managerial efficiency
EVTE ND ND ND +
directions and instrument monitoring and control can be ・Facilities where existing PCs can be used as client PCs
TVLG ND ND ND ND
performed from a personal computer (client PC) not
FVIE ND + + + connected to the instruments.
TLAF ND ND + + Moreover, client PC functions are performed on a server and
SALA + + + + client PCs corresponding to a Windows terminal service do
not need to install LabSolutions software.

Peptide LabSolutions server
Candidate FASTA format
Selection download via MRM Prediction Method Development Client PC Analysis equipment
UniProt Database via Skyline & Analysis room or an office

(x1,000) (x1,000) (x1,000) Area(x10,000) Area(x100,000)
453.80>628.40(+) 450.30>687.40(+) 376.10>392.80(+)
MRM1
1.00 453.80>279.10(+) 450.30>588.35(+) Pork 376.10>647.20(+)
MRM1
MRM1
MRM2
MRM2
MRM2
453.80>515.35(+) YDII Pork 450.30>475.25(+) LVVI 1.5 376.10>576.40(+) SALA Pork 7.5 1.5
MRM3 0.1% MRM3 0.1% MRM3 0.1% (A) (B)
0.75 YDII 1.5 LVVI
1.0 SALA 5.0 1.0
0.50 1.0
2.5 0.5
0.5
0.25 0.5
0.0 0.0
0.00 0.0 0.0 Acquisition
6.0 6.5 7.0 5.0 5.5 6.0 3.0 3.5 0.0 2.5 Conc. 0.0 2.5 Conc. controller PC UV FTIR RF ICPMS
(x1,000) (x10,000) (x1,000) Area(x10,000) Area(x10,000)
453.80>628.40(+) 450.30>687.40(+) 376.10>392.80(+) 7.5
MRM1
453.80>279.10(+) Pork 450.30>588.35(+) 376.10>647.20(+)
MRM1
MRM1
MRM2
MRM2
MRM2
4.0 453.80>515.35(+) YDII 1.0% 1.25 450.30>475.25(+) LVVI Pork 3.0 376.10>576.40(+) SALA Pork 1.5 (C) (D)
MRM3
MRM3
1.0%
MRM3
1.0%
YDII 1.00 LVVI
3.0 5.0
0.75 2.0 SALA 1.0 GCMS AA TOC
2.0
0.50 2.5
1.0 0.5
1.0
0.25
EDX Particle size
0.0 0.00 0.0 0.0 0.0 analyzer
6.0 7.0 5.0 5.5 6.0 3.0 4.0 0.0 2.5 Conc. 0.0 2.5 Conc. Combine with a Multi Data Registration license to enable
LC GC LC GC integrated management of these instruments’ data.
Source: Shimadzu Application News No. AD-0153
In summary, the established MRM-based method on a LC-MS/MS platform offers a highly sensitive and selective approach to
detect and monitor the presence of porcine-specific markers for semi-quantitation of the amount of pork in processed foods.
The established method from sample preparation to LC-MS/MS analysis exhibits excellent reliable sensitivity, and is potentially
able to support halal testing in processed food products. Analysis of various food matrices will be performed in the future.
Shimadzu’s Total Support for Food Safety
18 Analytical and Testing Instruments for Food Safety 19