Page 4 - Shimadzu Journal vol.3 Issue3

CONTENTS

Featuring Pharmaceutical Analysis

Insight from Customer
Professor Alexander (Leggy) Arnold from Milwaukee Institute for Drug Discovery 61
We interviewed Dr. Arnold, aka Leggy Arnold, an Associate Professor in the Department of Chemistry and Biochemistry,
Principle Investigator of the Shimadzu Laboratory for Advanced and Applied Analytical Chemistry and Founding Member
of the Milwaukee Institute for Drug Discovery (MIDD) at the University of Wisconsin-Milwaukee (UWM).

Drug Discovery
Metabolic Studies of Drug Candidates for Neurological Disorders and Asthma Based on 64
GABA A Receptor Subtype Selective Ligands using Mass Spectrometry
Development of pre-clinical experimental models to understand the in vivo metabolic performance of a drug is important in
the field of drug discovery. In the present study, an in vitro microsomal assay was designed to evaluate the metabolic stability
of GABAA receptor subtype selective ligands using microsomes and S9 fractions of human and mouse liver extracts. A
LC-MS/MS method was developed to quantify the amount of drug degrading over a period of time using verapamil as
internal standard.

Interview
The Challenge of Discovery and Development of Pharmaceutical Products 69
Using Imaging Mass Spectrometry: iMScope TRIO
Imaging mass spectrometry (IMS), which uses MALDI-TOF/MS analysis to identify the presence of target substances in
biosamples such as tissue samples, is a cutting-edge measurement technology that is becoming increasingly popular in
medical and biological research. We sat down with Hidefumi Kaji, PhD, of Mitsubishi Tanabe Pharma Corporation’s
DMPK (Drug Metabolism and Pharmacokinetics) Research Laboratories Research Division, and asked him about his use
of this technology.

Drug Discovery
Utility of High-resolution MALDI imaging in Drug Discovery: Histological Distribution of 72
Gentamicin in Proximal Renal Tubules of Rats
MS/MS imaging of gentamicin was performed from high-selectivity analysis of endogenous metabolites in biological tissue.
High-resolution (10 µm) imaging of rat renal cortex showed that gentamicin is specifically distributed in proximal renal tubules. A
new matrix application method is useful for sensitive MALDI imaging.

Posters from Recent Conferences 76
These articles were selected by Shimadzu. Relating to pharmaceutical analysis, they are from posters presented at recent
international conferences in 2015, such as ASMS and HPLC. They feature a variety of instruments we produce and
include cutting-edge technologies.

Topics
Renovated Showroom for Analytical & Measuring Instruments Newly Opened “Science Plaza” 78
Shimadzu Corporation has renovated its showroom for analytical & measuring instruments at its headquarters and
opened a new facility called “Science Plaza”.

Topics
Design Awards in 2015 79
Shimadzu wins two design awards this year.

Topics
SHIMADZU TECH TOUR at WCMic 2015 80
Shimadzu held a TECH TOUR at the 10th World Congress for Microcirculation and about 140 attendees from all over
the world joined it.

New Products
LCMS-8060, Py-Screener (Py-GC/MS Screening System for Phthalate Esters),
AOC-6000 (Autosampler for GCMS) 81