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Environmental




            Measuring Oil in Water - ASTM D5412

            The American Society for Testing and Materials (ASTM) testing standard D 5412 specifies testing for polycyclic aromatic hydrocarbons contained in
            water as oil. In this example, the target substance was separated from a solution prepared with a mixture of five polycyclic aromatic hydrocarbons
            using synchronized scan*. Fig. 1 is a fluorescence spectrum of the mixture of polycyclic aromatic hydrocarbones. Fig. 2 is a synchronized scan
            spectrum of Benzo[a]pyrene. Fig. 3 is a synchronized scan spectrum of the mixture. The fluorescence peak of Benzo[a]pyrene was not
            distinguished in the fluorescence spectrum of the mixture; however, it was clearly separated on the synchronized scan spectrum.

            * Synchronized scan
              Synchronized scan mode simultaneously scans samples using both an excitation monochromator and a fluorescence monochromator that are
              offset by fixed wavelength intervals. Sharp fluorescence peaks can be detected for target substances even if multiple types of components are
              mixed together.









                             Fluorescence Intensity













                           Fig. 1  Fluorescence Spectrum of the Mixture of Polycyclic Aromatic Hydrocarbons (Ex 300 nm)









                             Fluorescence Intensity             Fluorescence Intensity














                               Fig. 2  Synchronized Scan Spectrum of  Fig. 3  Synchronized Scan Spectrum of
                                        Benzo[a]pyrene (Offset 6 nm)    the Mixture (Offset 6 nm)



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