Page 5 - Shimadzu MALDImini-1

“Minimum” sample volume

MS analysis is possible over a wide mass range, even with microquantity samples
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By combining a MALDI ion source with Digital Ion Trap (DIT) technology, it is possible to carry out high-sensitivity MS and MS analysis even on
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micro-quantity samples. Not only can the mass of various molecules be checked, but it is also possible to carry out a wide range of analyses such
as identifying proteins and investigating the structure of glycans and glycopeptides.

Identifying protein digests

By carrying out MS and MS/MS measurements on protein digests, it is possible to determine the identity of a protein.
MASCOT MS/MS Ions Search
®
Mass spectrum

MS/MS @ m/z 1479 MS/MS @ m/z 2045

MS/MS @ m/z 1567 Sample volume: 500 fmol/spot
MS measurement conditions: m/z 650-5000, scan speed 4000 Da/s
MS/MS measurement conditions: default

Investigating glycopeptide structure
Through MS, MS/MS and MS measurements of synthetic O-linked glycopeptides, the glycan sequence can be found from MS/MS data and
3

3
the amino acid sequence of the peptide backbone can be found from MS data.
2676.3
MS

AHGVTSAPDTRK-FAM

MS/MS
Glycan sequence

MS 3
Peptide backbone

FAM-K
R T D P A S T V G H A
: Deoxyhexose
: Hexose
: N-acetylhexosamine
Sample volume: 50 pmol/spot
MS measurement conditions: mass range m/z 650-5000, scan speed 4000 Da/s
MS/MS and MS 3 measurement conditions: default

MALDImini-1
Matrix-Assisted Laser Desorption/Ionization Digital Ion Trap Mass Spectrometer 5