Page 7 - Shimadzu DPiMS-8060

PESI MS Solution Software Data Analyzed Using LabSolutions LCMS

PESI MS Solution software is used to control the probe, edit MS method files, and start measurements. The system can be used for a wide variety of applications, such as for library searches based on mass spectra obtained from product
Scan range and SIM settings are specified via the method editing window in LabSolutions LCMS software. Analysis can be started ion scans, for quantitative analysis based on ion chromatograms obtained from MRM, or for multivariate analysis using scan data.
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easily by selecting the probe control mode and MS method in PESI MS Solution. During analysis, the status can be confirmed in the Because the postrun analysis functionality in LabSolutions LCMS is used, data can also be easily compared to LC/MS/MS data.
LabSolutions LCMS window.

Control(C)
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CB1- CB1
2 CB1-
CB1-
t[2] 0 CB1-
CB1-
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PESI MS Solution Probe control method file LabSolutions method file Example of PCA Analysis t[1] Ellipse: Hotelling’s T2 (95%)
Library Search Results Window Model Mouse Group with Carbon Tetrachloride-Induced Acute Liver Damage and Control Group
Enables Use of LC/MS/MS Method Package Antidepressants 1 (0.05 to 10 ng/mL) Antidepressants 2 (1 to 44 ng/mL) Antidepressants 3 (0.1 to 10 ng/mL)
0.6 1 1.4
y = 0.0502x - 0.0059 y = 0.115x - 0.0039
0.5 R 2 = 0.9991 0.8 y = 0.0207x - 0.0249 1.2 R 2 = 0.9995
The DPiMS-8060 system uses a direct analysis method that does not involve liquid chromatography using probe electrospray 0.4 R 2 = 0.9967 1
0.6 0.8
ionization. MRM parameters can be specified based on transition information included in the LC/MS/MS method package. 0.3
0.4 0.6
Consequently, methods can be created easily using an Excel template. A key feature of PESI is the easy process flow from 0.2 0.4
0.1 0.2 0.2
pretreatment to measurement.
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0 2 4 6 8 10 0 5 10 15 20 25 30 35 40 45 0 2 4 6 8 10
Quantitative Analysis
Retention Time Quantitation Reference Compound
Compound (min) Polarity Ion Ion Type Calibration Curves for Three Types of Antidepressants
Amino acid
Amino acid
Amino acid Analysis System with No Carryover
Amino acid
Amino acid
Amino acid
Amino acid Only a tiny amount of sample attaches to the probe during each sampling, reducing the risk of contamination inside the mass
Amino acid spectrometer to near zero.
Amino acid
Amino acid Carryover can even be prevented by changing probes.
Standard substance Even concentrated samples or samples of unknown concentration can be measured directly without pretreatment.
Amino acid
Amino acid
Standard substance PPGT 50% 2-propanol/water 50mM NaCl
Amino acid
Amino acid
Amino acid

Mass Spectra After Repeatedly Switching Between Measuring PPGT and Blank Samples (PPGT 10 mg/mL)

DPiMS-8060
6 Kit for Direct Probe Ionization Mass Spectrometer 7