Page 5 - Shimadzu DPiMS-8060

DPiMS Can Be Used in Diverse Fields
™

Switch Easily Between PESI-MS and LCMS
Measures a Wide Variety of Samples Applicable for a Wide Variety of Objectives
• Body fluids, such as blood or urine • Detecting biological components The PESI TQ unit can be removed easily. The ability to exchange it with
• Tissue sections, such as from laboratory animals or foods • Detecting materials for chemical products an ESI unit means measurements can be performed using the same
• Plant materials, such as vegetables and fruits • Detecting drugs, poisons, etc. theory of ionization and standard substance, which allows comparing
data. The DPiMS-8060 was used for quantitative analysis of a forensic
sample from a person that took the drug MT-45. As a result, we were
able to obtain measurement data consistent with LCMS results in an
Measure a Variety of Samples with Only Simple Pretreatment extremely short time.

Rapid and Easy Drug Detection in Forensic Samples 10
• Place a piece of each organ on a biological sample plate and add 50 % ethanol (35 µL).
8
• Detection is possible by on-plate MRM / Product Ion Scan analysis. PESI-MS/MS LC-MS/MS
7.0 Inten. (×1,000) 181 MT-45 Standard 1.25 Inten. (×100,000) 181 Liver 6 Tissue Section (µg/mL) (µg/mL)
m/z 169 Area ratio (sample/IS) Liver 4.1 3.9
169 m/z 103 0.75 4 Brain 1.6 1.5
3.0 169 Heart 1.8 2.0
m/z 181 2 y = 0.0156x + 0.647 Lung 8.7 10.9
[M+H] + 0.25 [M+H] + R = 0.9991 Kidney 1.7 1.5
2
103 349 103 349 Measurement Time 0.5 min 20 min
100 200 300 400 m/z 100 200 300 400 m/z 0 0 50 100 150 200 250 300 350 400 450 500 550
Concentration [ng/mL] Quantitative Results of MT-45 in Forensic Sample
Calibration Curve for the Drug MT-45
8.0 Inten. (×1,000) 181 Lung 9.0 Inten. (×1,000) 169 181 Brain
This data was obtained from joint research with Mr. Kiyotaka Usui from Tohoku University Graduate School of Medicine.
169
4.0 5.0
Metabolomic Analysis Performed Easily
103 [M+H] + [M+H] + by Transferring LCMS Analytical Conditions to DPiMS Unit
349 103 349
1.0
100 200 300 400 m/z 100 200 300 400 m/z
In this example, transition information for metabolites (26 components) such
as amino acids, organic acids, and sugars were used for metabolomic analysis 0.4 DL-2-aminobutyric acid
3.0 Inten. (×10,000) 181 Kidney 1.25 Inten. (×10,000) 181 Heart of mouse livers. Metabolomes can be analyzed easily by customizing some of
169 0.2 taurine
2.0 the analytical conditions in the LCMS primary metabolite method package.
169 L-glutamic acid
0.75 The DPiMS-8060 system was used to measure the principal components in the 0 L-histidine
1.0 model mouse group with acute liver damage induced by carbon tetrachloride P[2] -0.2 L-leucine L-proline
[M+H] + Malic acid
103 349 0.25 103 [M+H] + and the control group. A significant difference was observed between model -0.4 L-glutamine
349
100 200 300 400 m/z 100 200 300 400 m/z and control groups (given p < 0.001 for Welch's t-test), based on the significant -0.6
contribution of taurine to the separation of groups in the PCA loading plot. L-ornithine
MT-45 Product Ion Scan Results for Each Organ
The difference was verified with a box-whisker plot. -0.8
-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8
p[1]
PCA Loading Plot
Analyzed Components (Blue: Positive; Red: Negative)
(c)
PESI-MS Measurement
3-hydroxybutyrate citric acid D-glucose 5
Collision Energy: 25 V Isocitric acid G6P glycine
Mass range: m/z 50 to 450 L-asparagine L-aspartic acid L-glutamic acid 4
Scan speed: 405 u/sec
Event time: 1 sec L-lactic acid L-malic acid L-serine Intensity (×10 6 ) 3
Desolvation line: 250°C pyruvic acid succinic acid taurine
Heat block: 50°C 2-aminobutyricacid L-glutamine L-histidine 2
Polarity: Positive L-leucine/L-isoleucine L-methionine L-ornithine ***
Biological Sample Plate Acquisition time: 0.5 min / event L-phenylalanine L-proline L-threonine 1
L-tryptophan L-tyrosine
This data was obtained from joint research with Mr. Kiyotaka Usui from Tohoku University Graduate School of Medicine. This data was obtained from joint research with Associate Professor Kei Zaitsu from Nagoya University Graduate School of Medicine. Box-whisker Plot of Taurine
DPiMS-8060
4 Kit for Direct Probe Ionization Mass Spectrometer 5