Page 5 - AXIMA Performance

AXIMA Performance™ -

Application-centric Solutions

Protein identification Biomarker discovery

Designed with the flexibility to adapt to users’ workflows: This exciting area encompassing clinical sample screening
from single sample manual acquisition to fully automated and profiling is comprehensively addressed using
data-dependent peptide mass fingerprinting (PMF) and automated acquisition methods and refined data
processing.
MS/MS for protein identification.
Data can be easily exported to third party software
• Peptide mass fingerprints are acquired and subjected to
packages to allow comparative experiments using a
an optional integrated Mascot® database search.
number of standard data formats including ASCII, mzXML
• User-defined acceptance limits for PMF-based protein
and mzData.
identification.
• Data-dependent MS/MS: using the results of the PMF Versatility
search, MS/MS may be performed on ions that were
matched to the top ranked protein hit (confirmation
• The system’s flexibility and uncompromised linear mode
MS/MS), in addition to those that did not (investigation
performance lends itself to alternative applications such
MS/MS). Batch searching of these MS/MS spectra is then
as quality control.
performed automatically to provide additional and
higher confidence protein identifications. • Launchpad™ software offers a module, Oligo Analysis™,
for performing fully automated QC analysis of large
• Data may be reprocessed and resubmitted for database
numbers of oligonucleotides, peptides or small
searching at a later time to provide additional information.
molecules, complete with a report indicating the
presence or absence of a target compound, an estimate
LC-MALDI of the purity and occurrence of known contaminants,
adducts or truncated/extended analogues.
• The AXIMA Performance provides total support for
• Polymer applications are fully catered for using our
LC-MALDI based experiments.
unique polymer analysis software, Polymer Analysis™,
• The software suite allows the fully automated suitable for use with both polymers and copolymers.
acquisition of LC separated samples deposited onto
MALDI targets and the subsequent identification of
proteins via MS/MS of the peptides detected.
• The workflow automatically provides a provisional %Int.
100 %Int.
intensity map of all sample spots across the target to 100
90 90 80
assess the distribution of peptides and identify the 70
80 60
position of the apex of the chromatographic peaks. 50
70 40
These are utilized to generate a candidate list and 60 30 20
10
MS/MS data is acquired for all discrete peptide ions. 50 0 80 100 120 140 160
m/z
• Exclusion lists are used to remove known contaminants 40
or high abundance peptides. 30
20
• All data is then subjected to an integrated Mascot® search. 10
• Low sample consumption allows multiple spectra to be 0 100 200 300 400 500 600 700 800 900 1000 1100
acquired from the same spot increasing the amount of m/z
MS/MS data obtained. Typical MS/MS spectrum obtained by automated acquisition
%Int. %Int.
100 100
90 90
80 80
70 70
60 60
50 50
40 40
30 30
20 20
10 10
0 0
3480 3485 3490 3495 3500 3505 3510 3515 3520 3525 1500 1520 1540 1560 1580 1600 1620 1640 1660
m/z m/z
MS spectrum of Insulin B chain demonstrating resolution Peptide MS spectrum demonstrating attomole level sensitivity
>20,000 (FWHM)